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    1year
    Comparison between Three Different Techniques for the Detection of EGFR Mutations in Liquid Biopsies of Patients with Advanced Stage Lung Adenocarcinoma. (PubMed, Int J Mol Sci)
    "Our results evidenced an equivalent detection ability between PCR-based techniques for circulating EGFR mutations. The NGS assay allowed detection of a wider range of EGFR mutations but showed a poor ability to detect T790M."
    Journal • Liquid biopsy
    |
    EGFR (Epidermal growth factor receptor)
    |
    EGFR mutation • EGFR L858R • EGFR exon 19 deletion • EGFR T790M • KIT mutation
    |
    therascreen® EGFR RGQ PCR Kit • Idylla™ ctEGFR Mutation Assay • Ion AmpliSeq™ Cancer Hotspot Panel v2
    over2years
    DIAGNOSTIC CONCORDANCE BETWEEN PCR-BASED AND NEXT GENERATION SEQUENCING METHODS IN DETECTING EGFR T790M MUTATIONS IN LIQUID BIOPSIES OF PATIENTS WITH ADVANCED STAGE LUNG ADENOCARCINOMA PROGRESSED AFTER TYROSINE KINASE INHIBITOR THERAPY (AIOM 2021)
    "Our results evidenced an equivalent ability in detecting EGFR T790M mutations between the PCR-based techniques (Therascreen® and Idylla™) investigated. The NGS assay allowed the detection of a wider range of EGFR and additional gene mutations, but showed a significantly poor ability to detect T790M. Table 1."
    Clinical • Liquid biopsy
    |
    EGFR (Epidermal growth factor receptor)
    |
    EGFR mutation • EGFR L858R • EGFR exon 19 deletion • EGFR T790M
    |
    therascreen® EGFR RGQ PCR Kit • Idylla™ ctEGFR Mutation Assay
    almost3years
    Evaluation of the Idylla ctEGFR mutation assay to detect EGFR mutations in plasma from patients with non-small cell lung cancers. (PubMed, Sci Rep)
    The Idylla system was able to detect the exon 19 deletion from 6 copies/mL and up to 91 copies/mL for the G719S mutation. These results support that the Idylla ctEGFR mutation assay is a rapid option for the detection of EGFR hotspots mutations in plasma samples, however a particular attention is needed for its interpretation.
    Journal • Clinical
    |
    EGFR (Epidermal growth factor receptor)
    |
    EGFR mutation • EGFR exon 19 deletion • EGFR T790M • EGFR G719S
    |
    Idylla™ EGFR Mutation Test • Idylla™ ctEGFR Mutation Assay