The 8-gene Lung Cancer Assay is the first lung cancer clinical testing assay based on Genetron One-Step Seq Method approved by NMPA, offering fast and easy-to-use testing procedures, that is suitable for independent operation within hospitals. With the Genetron One-Step Seq technology, the library construction process is finished in one step of reaction, minimizing manual operation to one mixture of reagents with DNA/cDNA. The chance of contamination in the process is minimal with the “sample in library out” workflow. The assay is compatible with both the Genetron S5 sequencing platform and the Genetron Chef System. The 8-gene Lung Cancer Assay covers EGFR, ALK, ROS1, BRAF, KRAS, HER2, MET, PIK3CA, and 7 genes of them are recommended biomarkers by the 2018 NCCN guideline for Non-Small Cell Lung Cancer (NSCLC) patients.