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AmoyDx® PIK3CA Five Mutations Detection Kit

The PIK3CA five mutation kit is a sensitive and selective real-time PCR assay for the detection of five mutations in the phosphoinositide-3-kinase catalytic subunit alpha (PIK3CA) gene. It employs mutation-specific primers and a PIK3CA-targeted proprietary fluorescent probe to detect low-copy number (1%) PIK3CA-mutant DNA in cancer tissue. The kit enables detection of the following mutations against a background of wild-type genomic DNA: H1047R（3140A>G), H1047L (3140A>T), E542K (1624G>A), E545D (1635G>T), E545K (1633G>A).