The EGFR Plus RGQ PCR Kit is a research use only assay for the qualitative detection and identification of mutations in exons 18, 19, 20 and 21 of the epidermal growth factor receptor (EGFR) gene using DNA extracted from plasma or formalin-fixed, paraffin-embedded (FFPE) tumor tissue. The kit is also intended for the semi-quantitative measurement of somatic mutations in exons 18, 20 and 21 of the EGFR gene using DNA samples extracted from plasma. The assay uses real-time PCR on the Rotor-Gene Q 5plex HRM instrument.