Neuroblastoma

We establish a robust, mutation-independent methylation-based liquid biopsy strategy for neuroblastoma that enables accurate, quantitative disease monitoring across all high-risk patients including those lacking trackable genomic alterations. This approach supports clinical translation of methylation-based cfDNA deconvolution as a broadly applicable platform for pediatric precision oncology.

Oncogene amplification and/or p53 pathway abnormalities and/or typical SCAs identify patients with intermediate-risk neuroblastoma with inferior outcome for whom intensified or alternative treatments should be considered.

Furthermore, regucalcin treatment suppressed metastatic activity, including the adhesion, invasion, and migration of neuroblastoma cells. Taken together, these results suggest that extracellular regucalcin may exhibit anticancer activity against SK-N-SH human neuroblastoma cells in vitro, offering a new strategy for neuroblastoma treatment.

P1, N=27, Not yet recruiting, Baylor College of Medicine | Initiation date: May 2026 --> Aug 2026

Furthermore, CYFIP1-overexpression in SH-SY5Y cells was associated with reduced basal neurite outgrowth and altered IL-6-associated neurite outgrowth. These findings suggest that CYFIP1-overexpression modifies cytokine-responsive transcriptional pathways in vitro and provides novel insight into how CYFIP1 dysregulation may contribute to dysregulated cytokine signaling in ASD, advancing our understanding of the molecular mechanisms underlying neuroinflammatory processes in this disorder.

Overall, our findings indicate that ICP27 differentially modulates apoptotic signaling depending on the cellular context, enhancing pro-apoptotic features in epithelial cells while limiting intrinsic pathway activation in neuron-like cells. This dual behavior underscores HSV-1 adaptability across microenvironments, with implications for viral pathogenesis and therapeutic targeting.

Moreover, pTAU and APP protein expressions were increased, while Nurr1 protein was decreased in reelin-silenced cells. These findings suggest that downregulation of reelin gene expression may contribute to neurodegeneration through alterations in topo IIβ and nurr1 expression, in addition to changes in proteins associated with AD pathology.

The results demonstrated that D-galactose- induced a more rapid and pronounced senescence phenotype in SH-SY5Y cells compared with hydrogen peroxide based on the molecular hallmarks of aging. Based on the study, it can be concluded that the D-galactose- induced model provides a more reliable and physiologically relevant in vitro model for investigating neuronal senescence .

Our results indicate that HSV-1-replicating SH-SY5Y cells produce not only virions but also cytotoxic protein aggregates containing poly-ubiquitin conjugates and alpha-synuclein. These findings may provide insight into mechanisms underlying virus-induced protein aggregation and cytotoxicity, which may be relevant to neurodegenerative processes.

Differentiation treatment of ADRN-type cell lines CHP-134, LAN-5, and SIMA resulted in neuron-like cells expressing synaptic marker and several neurotransmitter pathway genes. Our results revealed ADRN-type cell lines can be selected carefully based on molecular background, differentiation potential and neuronal gene expression for neurological disease modeling.

P1, N=12, Terminated, National Cancer Institute (NCI) | Completed --> Terminated; Drug supply issues

P2, N=286, Recruiting, Memorial Sloan Kettering Cancer Center | Trial completion date: Jun 2026 --> Jun 2027 | Trial primary completion date: Jun 2026 --> Jun 2027