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BIOMARKER:

FLT3 mutation + NPM1 mutation

i
Entrez ID:
3ms
Long-term remissions with Gilteritinib in early relapse after allogeneic stem cell transplantation of FLT3/NPM1 mutated acute myeloid leukemia. (PubMed, Blood Cell Ther)
Thus, FLT-3 inhibitors may be viable treatment options in this setting. Here, we report three patients with FLT3 and NPM1 mutated AML who relapsed early after allo-HSCT and were treated with gilteritinib (associated with donor lymphocyte Infusion in two patients) to achieve long-term remission without a second transplantation.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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NPM1 mutation • FLT3 mutation + NPM1 mutation
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Xospata (gilteritinib)
4ms
Early drivers of clonal hematopoiesis shape the evolutionary trajectories of de novo acute myeloid leukemia. (PubMed, medRxiv)
Thus, early CH-associated mutations that precede malignant transformation subsequently shape the evolutionary trajectories of AML through diagnosis, therapy, and relapse. DNMT3A , TET2 and ASXL1 mutations persist through AML-directed therapy Distinct CH-related mutations shape the evolutionary trajectories of AML from diagnosis through relapse.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1) • DNMT3A (DNA methyltransferase 1) • ASXL1 (ASXL Transcriptional Regulator 1) • TET2 (Tet Methylcytosine Dioxygenase 2) • SRSF2 (Serine and arginine rich splicing factor 2)
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FLT3 mutation • NPM1 mutation • DNMT3A mutation • ASXL1 mutation • TET2 mutation • CBL mutation • SRSF2 mutation • FLT3 mutation + NPM1 mutation
11ms
Clinico-Hematological Profile of Acute Myeloid Leukemia: Experience From a Tertiary Care Cancer Center in North India. (PubMed, Cureus)
The study highlights the presenting age is lower than global figures. The median time for initial diagnosis and the start of treatment is within the acceptable norms. Normal karyotype and NPM1 and FLT3 mutations were common in adult AML patients, whereas AML-ETO was more common in the pediatric cohort. These findings will help plan prospective studies and see the correlation with treatment outcomes. The laboratory workup practice currently complies with the standard guidelines at our center.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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FLT3-ITD mutation • FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
12ms
Fludarabine, Cytarabine, Granulocyte Colony-Stimulating Factor, and Idarubicin With Gemtuzumab Ozogamicin Improves Event-Free Survival in Younger Patients With Newly Diagnosed AML and Overall Survival in Patients With NPM1 and FLT3 Mutations. (PubMed, J Clin Oncol)
Overall, FLAG-Ida + GO significantly reduced relapse without improving OS. However, exploratory analyses show that patients with NPM1 and FLT3 mutations had substantial improvements in OS. By contrast, in patients with core binding factor AML, outcomes were excellent with DA + GO with no FLAG-Ida benefit.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
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cytarabine • Mylotarg (gemtuzumab ozogamicin) • daunorubicin • idarubicin hydrochloride • fludarabine IV
1year
Improved post-transplant outcomes in recent years for AML patients with FLT3-ITD and wild-type NPM1: a report from the EBMT acute leukemia working party. (PubMed, Clin Cancer Res)
In AML patients with FLT3 ITDand wild-type NPM1, we noticed a significant decrease over time in the CIR and improvement of LFS and OS, likely reflecting the efficacy of FLT-3 inhibitors, including when used as post-transplant maintenance, in this high-risk setting. On the contrary, no significant change over time was noticed in outcomes of patients harboring a FLT3 and NPM1 mutation.
Journal • Post-transplantation
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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NPM1 mutation • FLT3 mutation + NPM1 mutation • FLT3 wild-type
1year
Comparing Cairo Bishop and Howard Criteria for Identifying Clinically Meaningful Tumor Lysis Syndrome Among Acute Myeloid Leukemia Patients Treated with Venetoclax and Hypomethylating Agents: A Single Center Experience (ASH 2023)
56 pts received allopurinol ≥72 hours prior to starting ven, 21 received ≥2L of IV fluid per day starting at least the day prior to ven, and 56 had TLS labs measured every 8-12 hours during the first 3 days following ven initiation...We defined significant TLS as pts who required hospitalization (if started treatment outpatient) or ICU transfer, received rasburicase, developed AKI, or had treatment held due to TLS...Of the pts who developed significant TLS, a majority had spontaneous TLS; only 5 pts had significant TLS occur after starting ven. We also found that the Howard criteria for TLS better identify pts who will have adverse outcomes from TLS and may be a more clinically meaningful set of criteria to use in future studies.
Clinical
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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NPM1 mutation • FLT3 mutation + NPM1 mutation
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Venclexta (venetoclax)
1year
Combined Single Cell Flow Cytometry and Imaging Analyses Reveal Immunomodulatory Effects Exerted By Targeted Phospho-SYK Inhibitors with Elevated Sensitivity in NPM1 Mutated AML (ASH 2023)
Entospletinib (ENTO) and lanraplenib (LANRA) are inhibitors of spleen tyrosine kinase (SYK); the latter is a next-generation SYK inhibitor and is currently being evaluated in combination with gilteritinib (GILT) in patients with relapsed or refractory FLT3-mutated AML (NCT05028751)...Two different AML patient cohorts were assessed by ex vivo treatment with ENTO, LANRA and combinations of LANRA (trametinib, gilteritinib (GILT), aPD-1) over a range of time points (2h, 4h, 3d and 9d) and readouts for cellular phenotype (e.g., differentiation state, immune cell populations) and functional readouts (pSYK, viability) for each cohort were analyzed by HP-FC and sc-MI and associated with available mutation information (bulk gene and single cell RNA sequencing)...These support studies investigating the use of pSYK inhibitors in combination with other therapies to reduce tumor burden, and as an improved line of treatment for AML. Acknowledgements: We are appreciative of the patients and families for their time and participation.
Immunomodulating
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FLT3 (Fms-related tyrosine kinase 3) • MYC (V-myc avian myelocytomatosis viral oncogene homolog) • IDH1 (Isocitrate dehydrogenase (NADP(+)) 1) • IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2) • NPM1 (Nucleophosmin 1) • mTOR (Mechanistic target of rapamycin kinase) • SYK (Spleen tyrosine kinase)
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IDH1 mutation • IDH2 mutation • FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation • MLL mutation • SYK mutation
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Mekinist (trametinib) • Xospata (gilteritinib) • entospletinib (GS-9973) • lanraplenib (GS-9876)
1year
Safety and Efficacy Results from CLI120-001 a Phase 1 Study in RR-AML and HR-MDS: Update from Higher Dose Levels (ASH 2023)
In the ongoing phase 1 trial, RVU120 shows clinical activity in both AML and HR-MDS, inducing RBC transfusion independence and blast reduction with a tolerable safety profile. Clearance of BM blasts including a formal CR were observed in patients treated at different dose levels. Relevant target inhibition is achieved from 110 mg onwards with expected higher pSTAT 5 inhibition with further dose escalation.
Clinical • P1 data
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TP53 (Tumor protein P53) • FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
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RVU120
1year
Tuspetinib Myeloid Kinase Inhibitor Safety and Efficacy As Monotherapy and Combined with Venetoclax in Phase 1/2 Trial of Patients with Relapsed or Refractory (R/R) Acute Myeloid Leukemia (AML) (ASH 2023)
In an orthotopic mouse model of FLT3-mutant AML, tuspetinib exhibited greater antitumor activity than gilteritinib, entospletinib, venetoclax (VEN), and azacitidine (AZA) and combined favorably with VEN and AZA individually. Tuspetinib was well-tolerated and delivered single agent clinical responses across four dose levels among diverse AML genotypes; with a RP2D of 80 mg chosen for future single agent studies. Although early, the VEN/TUS combination has been well tolerated with preliminary objective responses noted.
Clinical • P1/2 data
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TP53 (Tumor protein P53) • FLT3 (Fms-related tyrosine kinase 3) • KIT (KIT proto-oncogene, receptor tyrosine kinase) • NPM1 (Nucleophosmin 1) • JAK1 (Janus Kinase 1) • SYK (Spleen tyrosine kinase)
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TP53 mutation • FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation • FLT3 wild-type
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Venclexta (venetoclax) • Xospata (gilteritinib) • azacitidine • entospletinib (GS-9973) • tuspetinib (HM43239)
over1year
UNLOCKING THE POTENTIAL OF SYNTHETIC PATIENTS FOR ACCELERATING CLINICAL TRIALS: RESULTS OF THE FIRST GIMEMA EXPERIENCE (EHA 2023)
These results demonstrate the success of this approach in producing a virtual dataset that perfectly mimics the original and that, from a "privacy by design" perspective, minimizes the risk of re-identification of patients. Mirroring an AML population treated with a conventional chemotherapeutic approach, synthAML1310 is suitable to represent the control group when testing novel innovative treatments, most likely in an in-silico randomizedtrial, but also in other settings like propensity score matching analyses in observational studies. Shifting to an in- silico trial would overcome the challenges of randomized trials and it would be beneficial also for patients.
Clinical
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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NPM1 mutation • FLT3 mutation + NPM1 mutation
over1year
Association of FLT3 and NPM1 mutations in patients with acute myeloid leukemia with metabolomic patterns determined by mass spectrometry. (ASCO 2023)
A preliminary model based on the targeted metabolomics approach was developed for the prediction of mutation status of NPM1 and FLT3 proteins in AML patients. Proposed model has a high fit value, validity, and strong predictive power. The reliability and validity of the model can be further increased by future multicenter studies.
Clinical • Metabolomic study
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
almost2years
GENOMIC LANDSCAPE OF MYELOID SARCOMA: A PLEA FOR BETTER MOLECULAR CHARACTERIZATION AND A ROAD MAP FOR TARGETED THERAPIES (EBMT 2023)
This comparative study sheds light on the unique molecular features of MS, pleading for the need to molecularly characterize all solid lesions occurring in AML context, and to explore targeted therapies in this high-risk category of patients.
Tumor mutational burden
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KRAS (KRAS proto-oncogene GTPase) • TMB (Tumor Mutational Burden) • FLT3 (Fms-related tyrosine kinase 3) • NRAS (Neuroblastoma RAS viral oncogene homolog) • NPM1 (Nucleophosmin 1)
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KRAS mutation • FLT3 mutation • NPM1 mutation • MTOR mutation • FLT3 mutation + NPM1 mutation
2years
Identification of a signature based on non-apoptotic regulatory cell death to improve prognosis prediction in acute myeloid leukaemia. (PubMed, Br J Haematol)
Specifically, patients with high NRG score may benefit from treatment with anti-EGFR and CDK2 inhibitors, including erlotinib and roscovitine. The NPM1 and FLT3 mutant cell lines undergo alterations after multiple drug treatments. Our established NRG signature and scoring highlight its vital clinical significance, emphasize the inevitability of stratifying treatment for different mutation subtypes and provide new ideas to guide personalized immunotherapy strategies for AML patients.
Journal • IO biomarker
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1) • CORIN (Corin, Serine Peptidase)
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FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
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erlotinib • seliciclib (CYC202)
2years
Comprehensive analysis of ID genes reveals the clinical and prognostic value of ID3 expression in acute myeloid leukemia using bioinformatics identification and experimental validation. (PubMed, BMC Cancer)
The bioinformatics analysis and experimental verification demonstrate that low ID3 expression independently affects OS and DFS in patients with CN-AML, which might be seen as a potential prognostic indicator in CN-AML.
Journal
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TP53 (Tumor protein P53) • FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1) • ID2 (Inhibitor Of DNA Binding 2) • ID3 (Inhibitor Of DNA Binding 3, HLH Protein) • ID1 (Inhibitor Of DNA Binding 1, HLH Protein)
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TP53 mutation • TP53 wild-type • NPM1 mutation • FLT3 mutation + NPM1 mutation
2years
Dexaml-02 : A Phase II Study of Dexamethasone Added to Induction and Postremission Therapy in Older Patients with Newly Diagnosed AML. a French Innovative Leukemia Organization (FILO) Study (ASH 2022)
Induction chemotherapy regimen consisted in one cycle of idarubicin 8 mg/m²/day, D1 to D5, cytarabine 100 mg/m²/d, CIV D1 to D7, lomustine 200 mg/m²/d, orally at D1 and dexamethasone 10 mg/12h, IV, D1 to D3...The addition of midostaurin in patients with FLT3-ITD or FLT3-TKD mutations was allowed during induction, consolidation and maintenance...Conclusion In older AML patients who are eligible for intensive treatment, adding dexamethasone to induction and consolidation chemotherapy is feasible and associated with a high response rate after a single induction cycle and encouraging overall survival. A historical comparison with the patient population of the LAM-SA 2007 trial (Pigneux A, JCO 2018) will be presented during the meeting to highlight a potential signal of activity.
Clinical • P2 data
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FLT3 (Fms-related tyrosine kinase 3) • ABL1 (ABL proto-oncogene 1) • BCR (BCR Activator Of RhoGEF And GTPase) • NPM1 (Nucleophosmin 1) • RUNX1 (RUNX Family Transcription Factor 1) • SRSF2 (Serine and arginine rich splicing factor 2) • RUNX1T1 (RUNX1 Partner Transcriptional Co-Repressor 1) • NR3C1 (Nuclear Receptor Subfamily 3 Group C Member 1)
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FLT3-ITD mutation • FLT3 mutation • NPM1 mutation • FLT3-TKD mutation • SRSF2 mutation • FLT3 mutation + NPM1 mutation
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cytarabine • Rydapt (midostaurin) • idarubicin hydrochloride • lomustine
2years
Long-Term Survival of Acute Myeloid Leukemia Responding Patients Who Stopped Azacytidine and/or Venetoclax Because of Poor Tolerance or Physician Choice: A Retrospective Multicenter Study from the French Innovative Leukemia Organization (FILO) (ASH 2022)
Our findings confirm the high efficacy of VEN-AZA treatment in AML. Discontinuation of VEN and/or AZA treatment in responders was associated with durable responses and survival. Strategies of de-escalation based on clinical response, MRD and/or molecular profiling may be envisaged but will require a prospective evaluation in clinical trials.
Retrospective data
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TP53 (Tumor protein P53) • FLT3 (Fms-related tyrosine kinase 3) • IDH1 (Isocitrate dehydrogenase (NADP(+)) 1) • NPM1 (Nucleophosmin 1)
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TP53 mutation • IDH1 mutation • FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
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Venclexta (venetoclax) • azacitidine
2years
Epag 2015 : A Phase II Randomized Placebo-Controlled Study to Assess the Impact on Outcome of Eltrombopag Administered to Elderly Patients with Acute Myeloid Leukemia Receiving Induction Chemotherapy. a French Innovative Leukemia Organization (FILO) Study (ASH 2022)
The induction chemotherapy regimen consisted of one cycle of daunorubicin 60 mg/m²/day (d), d1 to d3, cytarabine 100 mg/m²/d, CIV d1 to d7, lomustine 200 mg/m², orally d1...Pts then received 6 months of maintenance therapy alternating mercaptopurin and methotrexate. The addition of midostaurin in patients with FLT3-ITD or FLT3-TKD mutations was not allowed during induction...These differences are not statistically significant. Conclusion In older AML pts eligible for intensive treatment, adding eltrombopag to induction is feasible and safe, reduces significantly the need of platelet transfusions, yet did not impact survival in this study.
Clinical • P2 data
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FLT3 (Fms-related tyrosine kinase 3) • ABL1 (ABL proto-oncogene 1) • BCR (BCR Activator Of RhoGEF And GTPase) • NPM1 (Nucleophosmin 1)
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FLT3-ITD mutation • FLT3 mutation • NPM1 mutation • FLT3-TKD mutation • FLT3 mutation + NPM1 mutation
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cytarabine • Rydapt (midostaurin) • methotrexate • daunorubicin • lomustine • Promacta (eltrombopag)
2years
FLAG-Ida Combined with Gemtuzumab Ozogamicin (GO) Improves Event Free Survival in Younger Patients with Newly Diagnosed Acute Myeloid Leukaemia (AML) and Shows an Overall Survival Benefit in NPM1 and FLT3 mutated Subgroups. Results from the UK NCRI AML19 Trial (ASH 2022)
The MRC AML15 trial suggested a higher response rate and reduced relapse risk with FLAG-Ida compared to a Daunorubicin-araC (DA) +etoposide but did not show an overall survival (OS) benefit (Burnett, JCO,2013,31,3360). Furthermore this survival benefit was associated with a reduction in the requirements for transplant in CR1 and overall. Given the benefit observed in FLT3 mutated AML in the absence of a FLT3 inhibitor, studies combining FLAG-Ida-GO with Midostaurin are warranted.
Clinical
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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FLT3-ITD mutation • FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
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etoposide IV • Rydapt (midostaurin) • Mylotarg (gemtuzumab ozogamicin) • daunorubicin
2years
Prognostic impact of NPM1 and FLT3 mutations in patients with AML in first remission treated with oral azacitidine. (PubMed, Blood)
Median OS with Oral-AZA vs placebo was 28.2 vs 16.2 months, respectively, for FLT3mut/MRD- patients, and 24.0 vs 8.0 months for FLT3mut/MRD+ patients. In multivariate analyses, Oral-AZA significantly improved survival independent of NPM1 or FLT3 mutational status, cytogenetic risk, or post-IC MRD status.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
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Onureg (azacitidine oral)
2years
Prognostic Relevance of NPM1 and FLT3 Mutations in Acute Myeloid Leukaemia, Longterm Follow-Up-A Single Center Experience. (PubMed, Cancers (Basel))
Our data indicate that a high dose of ARAC plus idarubicin consolidation exerts a strong anti-leukemic effect in NPM1-mutated patients both with the FLT3 wild-type and mutated AML, while in the NPM1 wild-type and FLT3-mutated, the therapeutic effect remains unsatisfactory. New strategies incorporating target therapy with second-generation inhibitors will improve these results and their addition to this aggressive chemotherapeutic program merits testing.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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FLT3-ITD mutation • FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation • FLT3 wild-type • FLT3‐ITD  + NPM1 mutation
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cytarabine • idarubicin hydrochloride
over2years
New trial
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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NPM1 mutation • FLT3 mutation + NPM1 mutation
over2years
CLINICAL AND BIOLOGICAL MARKERS ASSOCIATED WITH LONG-TERM SURVIVAL FOR PATIENTS WITH ACUTE MYELOID LEUKEMIA (AML) IN REMISSION AFTER CHEMOTHERAPY IN THE QUAZAR AML-001 TRIAL OF ORAL AZACITIDINE (EHA 2022)
Conclusion Oral-AZA Tx was significantly associated with LTS vs PBO. In univariate analysis, Int-risk cytogenetics and NPM1 mut at Dx, and MRD response on-study, were significantly prognostic of LTS in both arms, whereas MRD– status at BL (post-IC) was associated with LTS only in the PBO arm.
Clinical • PD(L)-1 Biomarker • IO biomarker
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1) • CD8 (cluster of differentiation 8) • PD-1 (Programmed cell death 1) • HAVCR2 (Hepatitis A Virus Cellular Receptor 2) • CD4 (CD4 Molecule)
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FLT3 mutation • NPM1 mutation • PD-1 expression • CD8 expression • HAVCR2 expression • FLT3 mutation + NPM1 mutation
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Onureg (azacitidine oral)
over2years
THE ISTH-DIC SCORE PREDICTS 30-DAYS OUTCOME IN NON-M3 ACUTE MYELOID LEUKEMIA PATIENTS (EHA 2022)
A potential role of DIC score is to select patients who are at high-risk of fatal bleeding and therefore in need of aggressive transfusion support. Further studies on larger population are needed to confirm the findings, and to incorporate DIC score in prognostic models.
Clinical
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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NPM1 mutation • LDH elevation • FLT3 mutation + NPM1 mutation
over2years
RARE PHENOTYPE WITH CD33 ABSENT IN ACUTE MYELOID LEUKEMIA MIMICKING MRD POSITIVITY: A CASE REPORT (EHA 2022)
Treatment consisted of 2 induction cycles (cycle 1: 3 days of Daunorubicin 60 mg/m2 and 7 days of cytarabine 200 mg/m2; cycle 2: 6 days of cytarabine 1 g/m2 twice a day). Here we present a case report of AML with persistent absent CD33 expression since diagnosis that we found to be mimicking MRD positivity. Our data emphasizes the importance of clinical and technical expertise in MFC analysis and correlation of diagnostic and follow-up interpretation, especially in a heterogeneous disease such as AML, bringing up the need of standardization and constant training of the team.
Clinical • IO biomarker • Minimal residual disease
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FLT3 (Fms-related tyrosine kinase 3) • KIT (KIT proto-oncogene, receptor tyrosine kinase) • NPM1 (Nucleophosmin 1) • CD19 (CD19 Molecule) • RUNX1 (RUNX Family Transcription Factor 1) • CD38 (CD38 Molecule) • RUNX1T1 (RUNX1 Partner Transcriptional Co-Repressor 1) • CD123 (Interleukin 3 Receptor Subunit Alpha) • CD33 (CD33 Molecule) • CD34 (CD34 molecule) • PTPRC (Protein Tyrosine Phosphatase Receptor Type C) • NCAM1 (Neural cell adhesion molecule 1) • CD14 (CD14 Molecule) • ITGAM (Integrin, alpha M) • CD7 (CD7 Molecule) • ITGAX (Integrin Subunit Alpha X)
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NPM1 mutation • RUNX1 mutation • FLT3 mutation + NPM1 mutation • CD33 expression
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cytarabine • daunorubicin
over2years
Prognostic Relevance of DNMT3A, FLT3 and NPM1 Mutations in Syrian Acute Myeloid Leukemia Patients. (PubMed, Asian Pac J Cancer Prev)
Patients with FLT3-ITD and NPM1 mutations have the worst prognosis, where the presence of those mutations was significantly related to overall survival (OS) and EFS. Our study reflects that DNMT3A was not an extremely bad prognostic effect as an independent factor. We can declare according to this study that genetic mutation and variants detection could easily be incorporated into the regimen evaluation of AML patients.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1) • DNMT3A (DNA methyltransferase 1)
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FLT3-ITD mutation • NPM1 mutation • DNMT3A mutation • DNMT3A mutation + FLT3-ITD mutation • FLT3 mutation + NPM1 mutation • FLT3‐ITD  + NPM1 mutation • FLT3‐ITD + DNMT3A mutation
almost3years
FGF10/FGF17 as prognostic and drug response markers in acute myeloid leukemia. (PubMed, Curr Res Transl Med)
Our results indicated that FGF10 and FGF17 could be prognostic biomarkers for survivals of AML patients, and potential therapeutic targets for small-molecule inhibitors.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1) • FGF10 (Fibroblast Growth Factor 10) • MUC2 (Mucin 2) • ZRSR2 (Zinc Finger CCCH-Type, RNA Binding Motif And Serine/Arginine Rich 2)
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NPM1 mutation • FLT3 mutation + NPM1 mutation
3years
Impact of FLT3 internal tandem duplication and NPM1 mutations in acute myeloid leukemia treated with allogeneic hematopoietic cell transplantation. (PubMed, Cytotherapy)
In contrast to chemotherapy-only treatment, FLT3 and NPM1 mutational status does not appear to predict outcomes in patients with cytogenetically intermediate-risk AML following HCT. These results suggest that HCT may ameliorate the poor prognostic effect of FLT3 mutation and that HCT should be considered over chemotherapy-only treatment in FLT3-mutated AML.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
|
FLT3-ITD mutation • FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
3years
Distinct associations of NEDD4L expression with genetic abnormalities and prognosis in acute myeloid leukemia. (PubMed, Cancer Cell Int)
Our findings indicated that NEDD4L underexpression, as a frequent event in AML, was associated with genetic abnormalities and prognosis in AML. Moreover, NEDD4L expression may be involved in leukemogenesis with potential therapeutic target value.
Journal
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TP53 (Tumor protein P53) • FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
|
TP53 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
3years
Differential impact of IDH1/2 mutational subclasses on outcome in adult AML: Results from a large multicenter study. (PubMed, Blood Adv)
Among patients within the ELN2017 intermediate- and adverse-risk groups, RFS and OS were significantly better for patients with IDH2-R172K compared to wtIDH, providing evidence that AML with IDH2-R172K could be a distinct entity with a specific co-mutation pattern and favorable outcome. In summary, the presented data from a large cohort of IDH1/2 mutant AML patients indicate novel and clinically relevant findings for the most common IDH-mutation subtypes.
Clinical • Journal
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FLT3 (Fms-related tyrosine kinase 3) • IDH1 (Isocitrate dehydrogenase (NADP(+)) 1) • IDH2 (Isocitrate Dehydrogenase (NADP(+)) 2) • NPM1 (Nucleophosmin 1)
|
FLT3-ITD mutation • IDH1 mutation • IDH2 mutation • NPM1 mutation • IDH2 R172K • IDH1 R132C • FLT3 mutation + NPM1 mutation • IDH1 R132 • IDH2 R172
3years
Prognostic Impact of NPM1 and FLT3 Mutations at Diagnosis and Presence of Measurable Residual Disease (MRD) after Intensive Chemotherapy (IC) for Patients with Acute Myeloid Leukemia (AML) in Remission: Outcomes from the QUAZAR AML-001 Trial of Oral Azacitidine (Oral-AZA) Maintenance (ASH 2021)
An OS benefit was also observed with Oral-AZA vs PBO in pts in FLT3 mut at Dx, but outcomes may be confounded by co-occurring NPM1 mut , so further investigation is needed. MV analyses confirmed the independent prognostic influence of Oral-AZA, NPM1 and FLT3 mutations at Dx, cytogenetic risk at Dx, and post-IC MRD status on OS.
Clinical
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
|
FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
|
Onureg (azacitidine oral)
3years
[VIRTUAL] ABSENT CD33 IN AML PATIENT MIMICS MRD POSITIVE: A SINGLE CASE REPORT (HEMO 2021)
Methods Bone marrow (BM) sample was investigated for FLT3 and NPM1 mutation, RUNX1/RUNX1T1 and CBFB-MYH11 rearrangements, karyotype (KT) and immunophenotyping. Using an 8-colour antibody panel (CD45, CD34, CD117, CD33, HLA-DR, CD38, CD123, CD13, CD19, CD7, CD11b, CD4, CD56, CD64, CD11c, CD15, CD133, CD41a and NG2), we applied a gating strategy with fixed gates comparing normal to AML BM, settled to catch LAIPs (Leukemia Associated-Immunophenotypes), abnormal cell maturation pattern and Leukemic Stem Cell, that were detected on diagnosis and evaluated for MRD. Assays were performed at the Hematology Laboratory of the Medical School of Ribeirão Preto, University of São Paulo, in accordance with the Local Ethical Boards. Results A 21-year-old male patient with AML without maturation, after 60 days of symptoms, presented anemia (7.9 g/dL), thrombocytopenia (26 000/μL) and white blood cell count of 5710/μL with 73% blasts in peripheral blood and 84% in BM. MFC revealed CD45dim and low side scatter; positive staining for CD117, CD34, HLA-DR, CD38, CD123, CD11c, CD64, CD15 and CD133; negative staining for CD33, CD14, CD7, CD11b, CD4, CD56, CD19, CD41a and NG2. We found no metaphases (KT), no molecular abnormality and he failed risk stratification. Treatment consisted of 2 induction cycles (cycle 1: 3 days of Daunorubicin 60 mg/m2 and 7 days of cytarabine 200 mg/m2; cycle 2: 6 days of cytarabine 1 g/m2 twice a day) and 1 or 2 consolidation cycles ( 6 days of cytarabine 1 g/m2 twice a day). BM was obtained at diagnosis, 30 and 33 days after 1st and 2nd induction, and 3, 9, 12 and 15 months after consolidation of chemotherapy. The positivity of a specific phenotype (CD117+/CD34+/CD33wk/CD13+ ≥ 0.1%) was supported at diagnosis and follow-ups: 0.506%, 0.681%, 0.402%, 0.101%, 0.158% and 0.122%. Complete remission was achieved by day 30 after 1st induction and no change in the outcome was reported. Absent CD33 was observed in diagnosis and follow-ups, during and after treatment.Discussion Post-analytical phase of MFC is the most vulnerable to wrong interpretations. CD33 is a common myeloid antigen expressed on malignant blasts in AML and has been reported as a potential target therapy. CD33low blasts are associated to a more mature AML and its high expression is related to adverse landscapes, highlighting the importance of CD33 evaluation. Standard care for AML enrolls MRD monitoring and failure to achieve an MRD-negative, CR or detected MRD during or after therapy is associated with relapse or poor outcomes. Still, MRD analysis must embrace a set of standards in order to prevent post-analytic errors. Here we present a case report of AML with absent CD33 maintained from diagnosis to MRD follow-ups, mimicking MRD positivity. This data emphasizes the importance of caution in MFC analysis and correlation of diagnostic and follow-ups Results interpretation, as well as constant training of the team.
Clinical • IO biomarker • Minimal residual disease
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FLT3 (Fms-related tyrosine kinase 3) • KIT (KIT proto-oncogene, receptor tyrosine kinase) • NPM1 (Nucleophosmin 1) • CD19 (CD19 Molecule) • RUNX1 (RUNX Family Transcription Factor 1) • RUNX1T1 (RUNX1 Partner Transcriptional Co-Repressor 1) • CD123 (Interleukin 3 Receptor Subunit Alpha) • CD33 (CD33 Molecule) • CD34 (CD34 molecule) • PTPRC (Protein Tyrosine Phosphatase Receptor Type C) • NCAM1 (Neural cell adhesion molecule 1) • CD14 (CD14 Molecule) • ITGAM (Integrin, alpha M) • CD7 (CD7 Molecule)
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NPM1 mutation • RUNX1 mutation • FLT3 mutation + NPM1 mutation
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cytarabine • daunorubicin
3years
Mutations Synergize to Alter Epigenetic and Genomic Landscape of AML. (PubMed, Cancer Discov)
Flt3 and Npm1 mutations synergize to rewire the chromatin landscape in acute myeloid leukemia (AML).
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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NPM1 mutation • FLT3 mutation + NPM1 mutation
over3years
[VIRTUAL] MENIN INHIBITION DECREASES BCL-2 AND SYNERGIZES WITH VENETOCLAX IN NPM1/FLT3-MUTATED AML (EHA 2021)
Aims To investigate the anti-leukemic activity and potential synergism and mechanisms of the combination of the menin-MLL1 inhibitor SDNX-50469, an equipotent surrogate of the clinical compound SNDX-5613 and venetoclax in vivo in an NPM1c/FLT3-ITD/TKD patient-derived xenograft (PDX) model. Conclusion Our study further validated menin as a therapeutic target and demonstrated that its inhibition synergizes with venetoclax in NPM1/FLT3-mutated AML, which warrants further clinical evaluation. Inhibition of FLT3 may further enhance the therapeutic efficacy of menin and Bcl-2 co-targeting in NPM1 and FLT3 mutated AML.
IO biomarker
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FLT3 (Fms-related tyrosine kinase 3) • BCL2 (B-cell CLL/lymphoma 2) • NPM1 (Nucleophosmin 1) • CD38 (CD38 Molecule) • BCL2L1 (BCL2-like 1) • CD34 (CD34 molecule) • PTPRC (Protein Tyrosine Phosphatase Receptor Type C) • ITGAM (Integrin, alpha M)
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FLT3-ITD mutation • FLT3 mutation • NPM1 mutation • MLL rearrangement • MLL rearrangement • FLT3 mutation + NPM1 mutation
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Venclexta (venetoclax) • Revuforj (revumenib) • VTP-50469
almost4years
NPM1 and FLT3-ITD/TKD Gene Mutations in Acute Myeloid Leukemia. (PubMed, Int J Hematol Oncol Stem Cell Res)
The frequency of NPM1 and FLT3 mutations in this study was comparable to reports from Asian countries but lower than that reported from western countries. However, as the number of patients in the study was less, a larger number of patients need to be studied to corroborate these findings.
Journal
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FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1) • CD34 (CD34 molecule)
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FLT3-ITD mutation • FLT3 mutation • NPM1 mutation • FLT3 mutation + NPM1 mutation
almost4years
[VIRTUAL] CLINICAL OBSERVATION ON VENETOCLAX-BASED COMBINATIONS FOR PATIENTS WITH AML RELAPSING AFTER HSCT (APBMT 2020)
12 patients with AML relapsing after allo-HSCT were collected from January 2018 to may 2020.Five patients were treated with BCL-2 inhibitor (Venetoclax) combined with low-dose cytarabine (20mg/m2/day d1-14), 2 patients were treated with BCL-2 inhibitor combined with low-dose cytarabine and decitabine, 5 patients were treated with BCL2 inhibitor and azacytidine. BCL-2 inhibitor combined with chemotherapy is effective in the treatment of relapse of adult acute myeloid leukemia after allogeneic hematopoietic stem cell transplantation.
Clinical • IO biomarker
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TP53 (Tumor protein P53) • FLT3 (Fms-related tyrosine kinase 3) • NPM1 (Nucleophosmin 1)
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TP53 mutation • FLT3 mutation • NPM1 mutation • TP53 deletion • FLT3 mutation + NPM1 mutation • MLL fusion
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Venclexta (venetoclax) • cytarabine • azacitidine • decitabine