The VHIO-CARD-300 panel is a custom targeted capture assay compatible with formalin-fixed, paraffin-embedded derived genomic DNA samples. Over 430 cancer-related genes are enriched through specific capture with biotinylated probes, followed by sequencing in Illumina high or mid output instruments. The panel covers approximately 1.4Mb of exonic regions of pan-cancer-related genes for the study of their somatic single nucleotide variants and indels, as well as to study copy number alterations (CNA) for all of them