PredicineCNB is a companion LP-WGS assay to robustly estimate genome-wide copy number burden (CNB) from plasma and urine clinical samples, which can provide longitudinal disease monitoring in a cost-effective way. A comprehensive clinical study using PredicineCNB in 1,000 patients with prostate and bladder cancers demonstrated the promising clinical application of liquid biopsy-based genome-wide copy number changes in therapy monitoring.