Elypta's MIRAM Free Glycosaminoglycan Kit leverages established state-of-the-art lab methods for glycosaminoglycan (GAG) extraction and detection to deliver high and reliable performance in a standardized and scalable way. The method relies on an enzymatic digestion assay that depolymerizes GAGs into disaccharides. Disaccharides are subsequently labeled and separated by ultra-high performance liquid chromatography (UHPLC) and detected using electrospray ionization triple-quadrupole mass spectrometry (ESI-MS/MS) through multiple reaction monitoring.