Utilizing the sophisticated ARMS-PCR technique in tandem with a fluorescent probe, the LENA PAN-MPN qPCR Panel is designed for the in vitro qualitative detection of critical mutations in the JAK2, CALR, and MPL genes within genomic DNA. LENA PAN-MPN qPCR Panel is specifically engineered to detect and differentiate mutations including CALR TYPE1/TYPE2, MPL W515/S505N, and JAK2 V617F/K539L/exon 12 del, thereby aiding in the accurate clinical diagnosis of MPN. The process is straightforward and efficient: following DNA extraction, the sample is analyzed using a PCR machine, with results being available in approximately three hours.