ZEB1 expression

EIF5A overexpression and miR-491-5p suppression reversed NSCLC cell migration post circ-ZEB1 silencing. Our collective findings advised that circ-ZEB1 takes part in the malignant progression through regulating the miR-491-5p/EIF5A axis, highlighting its potential as an effective NSCLC therapeutic target.

And E-Cadherin overexpression reduced the migration and invasion ability of SKOV3 cells. SHP2 tyrosine phosphatase enhances the ovarian cancer cells' motility and invasiveness by upregulation of the integrin/E-Cadherin switch through ZEB1 signal.

In vivo studies using a xenograft mouse model of ovarian cancer revealed that tumors with higher levels of lncRNA DNM1P35 led to shorter survival, increased tumor burden, as well as elevated expression of proliferative marker Ki67 and EMT marker ZEB1. Our comprehensive study underscored the significance of lncRNA DNM1P35 in ovarian cancer progression, elucidating the underlying mechanism through miR-326/ZEB1 axis to promote ovarian cancer progression.

Both migration and invasion rate of the cancer cells transfected with METTL3-shRNA were significantly decreased. These findings highlight the pro-oncogenic function of METTL3 in colorectal and melanoma cancer cells, indicating that inhibiting METTL3 could be a promising approach for tumor suppression across multiple cancer types; nonetheless, further investigation is essential to confirm these observations.

Moreover, cell adhesion disappeared and cell morphology changed to a spindle shape, indicating sarcomatogenesis. In conclusion, this study reveals a mechanism for sarcoma development in OCS and provides novel therapeutic possibilities.

This study identifies CXCL5 and SLC6A14 as key biomarkers of MVI, highlighting their roles in tumor proliferation, immune resistance, and poor clinical outcomes. These findings provide valuable insights into the spatial organization of MVI and its contribution to ICC progression, offering potential therapeutic targets.

We demonstrate in our exploratory study that biomarkers involved in the process of EMT could have a prognostic impact in a cohort of patients with BC uniformly treated and with long-term follow-up. Genes known to be involved in EMT were associated with improved eribulin efficacy, while suggesting a poorer outcome with CDK4/6i.

The radiosensitivity was enhanced, with the radiosensitivity enhancement ratio being 1.37±0.11 vs 1.00±0.01 (P=0.037), and after radiotherapy the expression of γ-H2AX increased. Oct4 is involved in the regulation of epithelial-mesenchymal transformation of esophageal squamous cell carcinoma, which promotes the proliferation, migration, and radioresistance of esophageal squamous cell carcinoma.

GA-LCC-CEL nanoparticles represent a promising targeted drug delivery approach for breast cancer, enhancing CEL's antitumor efficacy and potentially inhibiting cancer progression by modulating EMT-related proteins.

Further investigations demonstrated that downregulation of ZEB1-AS1 induced epithelial-mesenchymal transition and increased sensitivity to cisplatin in Cal27 cells, while its upregulation reversed these effects, underscoring its pivotal role in tumor metastasis and cisplatin resistance in Cal27 cells...Our study underscored the critical role of ZEB1-AS1 in HNSC and its potential as a therapeutic target. By elucidating its functional mechanisms and utilizing a nucleus-targeting nanoparticle platform for efficient delivery, we proved the potential of ZEB1-AS1-targeted therapies in HNSC.

The present study focuses on the preparation of niosomes containing an extract of Anabasis setifera&evaluates their efficacy in inhibiting the growth&proliferation of HeLa.Thin-layer hydration technique was used to prepare niosomes/extract nanoparticles(NPs).The physicochemical properties of the synthesized NPs were confirmed by SEM,DLS,zeta potential analysis&FTIR.The cytotoxicity of free extract,free niosome&NPs was investigated by MTTassay.For this purpose,solutions of the three mentioned agents were prepared&diluted in 400,200,100,50,25,12.5&6.25µg/ml concentrations&incubated for24,48&72 hours.After calculating the IC50 concentration&treating the cells with this concentration,Real-time-PCR(to measure changes in the expression of apoptosis and metastasis genes),flowcytometry(to determine the amount of early and late induced apoptosis)&cellcycle test(to determine the stopping stage of the cancer cell division cycle)were performed.Also,the scratch test(The ability to inhibit cell metastasis after treatment)was used to evaluate cell migration.The MTTassay results showed that 72hours of treatment with NPs has the greatest effect on the death of cancer cells.Real-time PCR showed that the expression of the Bad gene increased dramatically and the expression of the BCL-XL,ITGA5&ZEB-1 decreased significantly.The flowcytometry results showed that 48.64% of HeLa cells underwent apoptosis after treatment with synthesized NPs.The scratch test results showed that cancer cell metastasis stopped after treatment with NPs.The research demonstrates the significant potential of NPs,as highly efficient drug carriers for cancer therapy.

A positive feedback loop is formed among FUS, circZEB1, miR-128-3p and LBH in GSCs. Our study uncovers a critical role of circZEB1 and provides a novel biomarker for treating GBM.