TYRP1 (Tyrosinase Related Protein 1)

Our results demonstrate that exogenous PACAP modulates the expression of multiple target molecules in melanoma cells. Some of the significantly responding molecules take part in hyaluronan homeostasis, suggesting an effect of PACAP on tumour matrix composition, through which it can modulate invasiveness of melanoma cells.

These findings support the therapeutic potential of O. aurita for pigmentary disorders and skin health. Further studies should investigate its efficacy and safety in vivo and explore its application in cosmeceutical and nutraceutical formulations.

The proteasome inhibitor MG132 confirmed that fisetin accelerates β-catenin and MITF degradation. Additionally, inhibition of the PI3K/AKT pathway by LY294002 or the ERK pathway by PD98059 reversed fisetin's reduction of tyrosinase activity and melanin synthesis, further verifying the participation of these pathways. Computational docking integrated with deep learning-based CNN scoring revealed that fisetin interacts with PKCα, β-catenin, tyrosinase, and TYRP1. Collectively, these findings suggest that fisetin exerts multi-targeted inhibitory effects on melanogenesis, highlighting its potential as a therapeutic and cosmetic agent for hyperpigmentation.

This study reveals a broader germline landscape in early-onset PCa among men of African descent. Inherited immune-metabolic dysfunction may contribute to tumorigenesis through microenvironmental dysregulation, highlighting novel pathways for investigation in larger and ancestrally diverse cohorts.

NJVO effectively reversed SP/CORT-induced hyperpigmentation by suppressing the activity and expression of TYR and TRPs and inhibiting melanosome maturation in mouse B16F10 melanoma cells.

Collectively, these findings demonstrate that CBN effectively modulates the melanogenesis signaling pathway by targeting both upstream kinases and downstream melanogenic genes. These findings suggest that CBN holds great promise as a bioactive agent for skin-whitening applications and warrants further research to confirm its clinical efficacy and safety.

Female C57BL/6N mice were subjected to hair cycle synchronization through depilation, followed by cyclophosphamide (CYP) administration to induce hair loss and graying...Moreover, propolis upregulated expressions of Lef1 and melanogenic genes (Tyr, Tyrp1, Dct) at 30 days of treatment. These findings suggest that Philippine stingless bee propolis promotes hair follicle regeneration and melanocyte function, offering a potential natural therapeutic approach for CIA.

Notably, the EPE-induced depletion of tyrosinase and TRP-1 was prevented by joint exposure to EPE and the proteasome inhibitor MG132...Immunoprecipitation analysis further revealed that EPE induces the ubiquitination of tyrosinase and TRP-1. Taken together, these results suggested that EPE induces proteasomal degradation of tyrosinase and TRP-1 by enhancing the ubiquitination of these targets, leading to the depletion of these proteins and the inhibition of melanogenesis.

It not only identified potential therapeutic targets but also emphasized the importance of understanding the broader implications of targeting these proteins, including potential adverse effects. The results lay the groundwork for further exploration of personalized treatment strategies for CM.

The resulting Mel-A not only possesses good photothermal stability and biocompatibility, but also displays higher photothermal conversion efficiency (66.5%) with good photoacoustic imaging (PAI) performance, significantly enhancing the efficacy of photothermal therapy of breast tumor. The study provides a promising strategy to regulate biosynthesis of melanin nanoparticles for improving their applications in cancer diagnosis and therapy.

Binding affinity analysis revealed that some derivatives exhibited comparable or superior docking scores to commercial inhibitors such as Imatinib...Among the tested ligands, compound 1d demonstrated exceptional binding affinity and stability across multiple targets, particularly the A2A adenosine receptor and TYRP1, which are highly related to epigenetic regulation and cancer progression. These findings suggest that triazole derivatives could serve as promising anticancer agents, warranting further experimental validation to assess their therapeutic potential.

Parallel experiments in B16F10 cells confirmed cross-species melanin suppression, with reduced gene expression and melanin synthesis-related protein (Mitfa and Tyr) at subtoxic concentrations (0.5 and 2.5 mg/L), which was also verified by Masson-Fontana staining. These findings demonstrated that 6PPD disrupted conserved melanogenic pathways by targeting enzymatic activity, posing ecological risks to aquatic and terrestrial organisms.