TRG (T Cell Receptor Gamma Locus)

The preferential enrichment of TCRG rearrangements in pulmonary cases compared with gastric cases highlights site-specific immune milieus shaping T-cell clonality. Together with distinct B-T spatial interaction patterns, these findings support dynamic B-T cell crosstalk and potential multilineage evolution within the MALT lymphoma microenvironment.

EBV-RLH generally followed an indolent course; however, it may coexist with hematologic malignancies or develop after multichemotherapy. Careful histopathologic evaluation is essential to avoid overlooking concurrent malignancy or unnecessary treatment.

A moderate concordance was found between histology and molecular clonality. Results from this molecular clonality emphasize the importance of interpreting data in association with histopathological features of the lesions.

This cohort study's analysis of TCRB and TCRG repertoires identified specific clonotypes that were associated with more aggressive subtypes and poorer survival in patients with MF/SS. Incorporating TCR sequencing into clinical practice may enhance risk stratification, enabling earlier identification of high-risk patients who could benefit from closer monitoring and timely implementation of more intensive treatment strategies in the disease course to improve clinical outcomes.

This study developed a novel, robust TRGPI that accurately predicts patient prognosis and immunotherapy responses in LUAD, providing a valuable tool for precision medicine and personalized treatment strategies.

Three months after rituximab monotherapy, bilateral pleural effusions developed...This case demonstrates phenotypic and clonal diversity of monoclonal B/PC-Ps in TFHL, which can obscure the neoplastic TFH proliferation. In such cases, careful clinicopathological correlation and the integration of immunophenotypic and genetic analyses may aid in the diagnosis.

The incidence of T-LGLL is low. The initial symptoms of T-LGLL include anemia, fatigue, lymph node enlargement, splenomegaly, and higher percentage of lymphocytes in peripheral blood, the percentage of abnormal T cells in bone marrow was significantly increased. Analysis of flow cytometric immunophenotyping, TCR gene rearrangement, and hot spot genes such as STAT3 and STAT5b, can improve the diagnostic accuracy.

TRG-AS1 is a potential predictor of poor prognosis in gastric cancer patients and targets miR-873-5p to inhibit the progression of cancer.

Cutaneous lymphocytosis was diagnosed through histopathological examination and immunohistochemical investigation. Clonal rearrangement of the T-cell receptor gamma locus supports that lymphocytosis in this case was an indolent lymphoma.

Lastly, IPS, TCIC, TIDE, and IMvigor210 cohort analysis illustrated that the immunotherapy response may be accurately predicted using TCRG. A TCRG classifier is an excellent resource for predicting a patient's prognosis, potentially guiding the preservation of laryngeal function, and identifying patients who may have a positive response to immunotherapy, which might have profound effects on therapeutic practice.

In conclusion, our study identified TCRGs, constructed and validated a TCRS for OS, and assessed immune response and drug sensitivity in different risk groups of OS patients. These findings provide novel insights into personalized treatment strategies for OS, potentially guiding more effective therapeutic interventions.

Here we describe an integrated approach to assess clonality via analysis of Ig heavy chain (IGH), Ig kappa (IGK), TCR beta (TRB), and TCR gamma (TRG) gene rearrangements, based on the standardized multiplex PCRs as originally developed by the European BIOMED-2 consortium (currently named EuroClonality). The described protocol covers the pre-analytical phase of DNA isolation (from formalin-fixed paraffin-embedded and fresh tissues, body fluids, peripheral blood, and bone marrow), the analytical phase of PCR GeneScan analysis, and the post-analytical interpretation of the obtained profiles, following established guidelines.