PPFIBP2 (PPFIA Binding Protein 2)

LINC00519 upregulation may serve as a potential indicator of CCA aggravation. LINC00519 promoted CCA development by targeting miR-876-3p to affect cancer cell activities. GATA1, a potential target of miR-876-3p, suggests the potential underlying molecular pathway. Targeting LINC00519 could be an efficient way to manage CCA.

Importantly, pharmacological inhibition of YAP1 using CA3 led to a notable decrease in EMT markers, invasion, and migration, suggesting that blocking the YAP1-PPFIBP2 axis may serve as an effective therapeutic strategy in HNSCC. In conclusion, our study identifies the YAP1-PPFIBP2 interaction as a crucial mediator of tumor aggressiveness in HNSCC, offering new insight into metastatic progression and highlighting a promising target for therapeutic intervention.

MK13 resulted in a strong reduction (about 80%) in viability, especially against CCLP1 cells when compared with doxorubicin...Apoptosis was confirmed at molecular level with the upregulation of BAX, a pro-apoptotic BH-3 only protein, and DR5, a cell surface death receptor. MK13 seems to be a promising anticancer compound against iCCA and deserves further attention for in vivo proof-of-concept studies.

Additionally, several unidentified metabolites were detected, indicating the potential to discover novel metabolic variations. These findings establish our method as a robust tool for single-cell spatial metabolomics with broad applicability in studying complex cellular interactions and advancing both research and clinical applications.

The profile of GS derivatives regulates the stem-like properties of iCCA cells.

Both individual gene and gene-set analyses identified candidates associated with LPCa. The novel association of PPP1R3A and LPCa risk merits further investigation.

Moreover, PRC1 was found to exert regulatory control over glycolysis and the mammalian target of rapamycin complex 1 (mTORC1) pathway in the context of CHOL based on KEGG and GSEA analysis. Collectively, these results underscore the pivotal role of PRC1 in CHOL progression, wherein it modulates glycolysis and the mTORC1 pathway under the regulatory influence of FOXM1.

rhTHBS2 was more intense in inducing invasiveness and in committing the HuCCT-1 cells to a mesenchymal-like phenotype and was therefore a stronger enhancer of the malignant behavior of iCCA cells compared to rhTHBS1. Our data extend the role of THBS1 and THBS2, which are not only able to hinder the vascular network and promote tumor-associated lymphangiogenesis but also exacerbate the malignant behavior of the iCCA cells.

CTGF was associated with tumor-stroma crosstalk in BTC. CTGF activated stromal SPARC expression, which promoted tumor progression, particularly at the invasion front. SPARC expression at the invasion front after NAC-RT may serve as a prognosis predictor.

NSG mice were injected with CCLP1 spheres and treated with the FASN inhibitor orlistat (240mg/Kg)... FA promote malignant features of CCA and increase CSC markers. FASN expression levels correlate with survival in patients with CCA and promote CCA growth in mice. Lipid metabolism could be a new target to block CCA progression.

NSG mice were injected with CCLP1 spheres and treated with the FASN inhibitor orlistat (240mg/Kg)... FA promote malignant features of CCA and increase CSC markers. FASN expression levels correlate with survival in patients with CCA and promote CCA growth in mice. Lipid metabolism could be a new target to block CCA progression.