PDIA3P1 (Protein Disulfide Isomerase Family A Member 3 Pseudogene 1)

In addition, wilms tumor 1-associated protein (WTAP) mediated m6A modification enhances the stability of PDIA3P1 through Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) dependent recognition. Taken together, these findings reveal the key role of PDIA3P1 regulates glycolysis-H4K8la-BMP7 axis in the progression of ESCC and provides new insights into the interplay between metabolic reprogramming and epigenetic regulation.

Our research revealed a novel mechanism by which a positive feedback loop exists between PDIA3P1 and OCT4. It also demonstrated that the PDIA3P1-WWP2-OCT4 loop is beneficial for promoting the cancer stem cell properties of ESCC. Owing to this regulatory relationship, the PDIA3P1-WWP2-OCT4-positive feedback loop might be used in the diagnosis and prognosis, as well as in the development of novel therapeutics for esophageal cancer.

Furthermore, doxorubicin and topotecan were considered to hold the most promising therapeutic potential robustly in silico evidence and in vitro experiments for HNSCC patients in the high-risk subtype. Our discovery revealed that the 10 mA-associated prognostic pseudogenes significantly contribute to predicting immunotherapy benefits and therapeutic agents, which might bring some potential implications for both immunotherapy and chemotherapy in HNSCC.