News - PCNA expression

Bacillus coagulans MF-06 alleviates intestinal mucosal barrier from damage in chicks infected with Salmonella pullorum via activating the Wnt/β-catenin pathway. (PubMed, Front Microbiol)

Analysis through 16S rRNA sequencing revealed that the intake of Bacillus coagulans MF-06 led to a significant decrease in the relative abundance of Lachnoclostridium, Shuttleworthia, and unidentified-Eggerthellaceae (p < 0.05). Collectively, the Bacillus coagulans MF-06 may provide a protective effect against Salmonella pullorum infection in chicks by enhancing growth performance, strengthening the integrity of the intestinal mucosal barrier, and stabilizing the gut microbiota.

2 days ago

Journal

MYC (V-myc avian myelocytomatosis viral oncogene homolog) • CLDN1 (Claudin 1) • PCNA (Proliferating cell nuclear antigen) • MUC2 (Mucin 2) • TJP1 (Tight Junction Protein 1) • CLDN2 (Claudin 2) • OCLN (Occludin)

PCNA expression

YAP Promotes Chemoresistance to 5-FU in Colorectal Cancer Through mTOR/GLUT3 Axis. (PubMed, J Cancer)

This study validated the role of YAP as an oncogene in CRC, as it promoted chemoresistance through the mTOR/GLUT3 axis. These results suggested YAP as a potential target for promoting the efficacy of chemotherapy in patients with CRC.

6 days ago

Journal

PCNA (Proliferating cell nuclear antigen)

PCNA expression

5-fluorouracil

16d

Ferroptosis inducer Erastin inhibits proliferation of liver cancer cells in vitro by downregulating ACSL4 (PubMed, Nan Fang Yi Ke Da Xue Xue Bao)

ACSL4 level is significantly overexpressed in liver cancer. Erastin increases MDA contents and down-regulates ACSL4 expression, thereby promoting ferroptosis and inhibiting proliferation of liver cancer cells, and these effects can be reversed by Fer-1.

16 days ago

Preclinical • Journal

ACSL4 (Acyl-CoA Synthetase Long Chain Family Member 4) • PCNA (Proliferating cell nuclear antigen)

PCNA expression

erastin

17d

Synergistic inhibition of proliferation and induction of apoptosis in oral tongue squamous cell carcinoma by mebendazole and paclitaxel via PI3K/AKT pathway mitigation. (PubMed, Naunyn Schmiedebergs Arch Pharmacol)

Both MBZ and paclitaxel treatments inhibited of cell proliferation and microtubule formation by reducing the PI3K/AKT pathway in CAL-27 and UM-SCC-1 cells, with the combination demonstrating synergistic effects. Our study suggests MBZ and paclitaxel as potential agents for the treatment of OTSCC.

17 days ago

Journal • PARP Biomarker

CASP3 (Caspase 3) • PCNA (Proliferating cell nuclear antigen)

PCNA expression

paclitaxel • mebendazole

21d

Genotoxic and Anti-Genotoxic Assessments of Fermented Houttuynia cordata Thunb. Leaf Ethanolic Extract and Its Anti-Cancer Effect in a Dual-Organ Carcinogenesis Model of Colon and Liver in Rats. (PubMed, Foods)

Its mechanisms of action may involve anti-inflammatory effects, the prevention of genotoxicity, the modulation of xenobiotic-metabolizing enzymes, and the inhibition of cancer cell proliferation. These findings support the use of FHCL as a natural supplement for preventing cancer.

21 days ago

Preclinical • Journal

PCNA (Proliferating cell nuclear antigen)

PCNA expression

23d

GH inhibits ALV-J replication and restricts cell cycle by activating PI3K/Akt signaling pathway. (PubMed, Poult Sci)

More importantly, we found that GH restricted cell proliferation and apoptosis, and inhibited the replication of ALV-J by activating the PI3K/Akt signaling pathway in DF-1 cells. In conclusion, these results indicate GH plays a role in the antiviral response against the replication of ALV-J, providing evidence of an interaction between GH and the innate immunity in chickens.

23 days ago

Journal

CCND1 (Cyclin D1) • CASP3 (Caspase 3) • CASP8 (Caspase 8) • CCND2 (Cyclin D2) • PCNA (Proliferating cell nuclear antigen) • CCNB2 (Cyclin B2) • CDKN1A (Cyclin-dependent kinase inhibitor 1A) • IFNA1 (Interferon Alpha 1) • ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1) • IFNB1 (Interferon Beta 1)

CCND1 expression • PCNA expression

28d

Pyrotinib induces cell death in HER2-positive breast cancer via triggering HSP90-dependent HER2 degradation and ROS/HSF-1-dependent oxidative DNA damage. (PubMed, Cell Stress Chaperones)

Furthermore, the in vivo results demonstrated a pronounced anticancer effect of pyrotinib in the SKBR3 xenograft mouse model, concomitant with a reduction in HER2 expression. In summary, our findings provide novel insights into the mechanism of pyrotinib in the treatment of HER2+ BC.

28 days ago

Journal

HER-2 (Human epidermal growth factor receptor 2) • PCNA (Proliferating cell nuclear antigen) • HSP90AA1 (Heat Shock Protein 90 Alpha Family Class A Member 1Heat Shock Protein 90 Alpha Family Class A Member 1)

HER-2 positive • HER-2 expression • PCNA expression

Irene (pyrotinib)

28d

Effects of Peiminine on biological behavior and chemotherapy resistance of ovarian cancer by regulating the FOXO3-FOXM1 signaling axis (PubMed, Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi)

Methods Ovarian cancer cells SKOV3 and SKOV3/cisplatin (DDP) were used as research objects...SKOV3/DDP cells were divided into control group (normal culture), DDP group (20 μg/mL DDP), L-PMI group, M-PMI group, H-PMI group (20 μg/mL DDP+50, 100, 200 μmol/L PMI), and carbenoxolone (CBX) group (20 μg/mL DDP+200 μmol/L PMI+50 ng/mL CBX)...The number of clonal cells, invasive cells, and migrating cells, the protein expressions of PCNA, MRP5 and FOXM1 in CBX group were higher than those in H-PMI group, and the apoptotic rate, the protein expressions of Caspase-3 and FOXO3 in CBX group were lower than those in H-PMI group. Conclusion PMI may activate FOXO3 and inhibit FOXM1, thereby suppressing the proliferation, invasion, migration, and chemotherapy resistance of ovarian cancer cells.

28 days ago

Journal

CASP3 (Caspase 3) • FOXM1 (Forkhead Box M1) • FOXO3 (Forkhead box O3) • PCNA (Proliferating cell nuclear antigen)

PCNA expression

cisplatin

30d

The role of peritumoral electroacupuncture in regulating cuproptosis for sensitization of chemotherapy efficacy in mice with triple-negative breast cancer (PubMed, Zhen Ci Yan Jiu)

The combination of PEA and DOX can effectively control the growth rate of tumors in mice with TNBC, which may contribute to its function in enhancing the chemotherapy efficacy of DOX on TNBC by promoting cuproptosis.

30 days ago

Preclinical • Journal

PCNA (Proliferating cell nuclear antigen) • DLAT (Dihydrolipoamide S-Acetyltransferase) • FDX1 (Ferredoxin 1) • LIAS (Lipoic Acid Synthetase)

PCNA expression

doxorubicin hydrochloride

N6-methyladenosine-modified circRPS6KC1 regulated cellular senescence in prostate cancer via FOXM1/PCNA axis. (PubMed, Cell Signal)

FOXM1 mediated the transcription of PCNA and influenced the p21 degradation, which resulted in up-regulation of p21 protein in a p53-independent manner. In conclusion, our findings showed that N6-methyladenosine modification by METTL3 and YTHDF1 stabilized circRPS6KC1, and circRPS6KC1 played an essential role on cellular senescence via FOXM1/PCNA axis in prostate cancer.

1 month ago

Journal

FOXM1 (Forkhead Box M1) • PCNA (Proliferating cell nuclear antigen) • CDKN1A (Cyclin-dependent kinase inhibitor 1A) • METTL3 (Methyltransferase Like 3) • MIR761 (MicroRNA 761)

PCNA expression

2ms

GRK2 Protein Mediates the ANRIL, a lncRNA, to Affect the Proliferation and Apoptosis of Kasumi-1 Cells. (PubMed, Front Biosci (Landmark Ed))

The GRK2 protein-mediated ANRIL, increasing Kasumi-1 cell proliferation and inhibiting apoptosis by activating the PI3K/AKT/BCL-2 pathway.

2 months ago

Journal • IO biomarker

BCL2 (B-cell CLL/lymphoma 2) • CDKN2A (Cyclin Dependent Kinase Inhibitor 2A) • CASP3 (Caspase 3) • PCNA (Proliferating cell nuclear antigen)

BCL2 expression • BAX expression • PCNA expression

2ms

Hypoxia-dependent recruitment of error-prone DNA polymerases to genome replication. (PubMed, Oncogene)

Our results suggest that the tumor microenvironment can lead the cell to forgo, to some extent, the fast and accurate canonical DNA polymerases, for the more flexible and robust, but low-fidelity TLS DNA polymerases. This might endow cancer cells with resilience to overcome replication stress, and mutability to escape the immune system and chemotherapeutic drugs.

2 months ago

Journal

HIF1A (Hypoxia inducible factor 1, alpha subunit) • PCNA (Proliferating cell nuclear antigen)

HIF1A expression • PCNA expression

2ms

Correlation of magnetic resonance imaging and computed tomography with biological factor expression and lymph node metastasis in aggressive prostate cancer. (PubMed, Urol Int)

MRI and CT manifestations of aggressive PCa had certain characteristics; MRI manifestations and qMRI possessed a correlation with biological factors and LNM; ADC could be employed to assess the aggressiveness of PCa.

2 months ago

Journal • MRI

PCNA (Proliferating cell nuclear antigen)

CDKN1B expression • PCNA expression

2ms

Key Genes Involved in the Beneficial Mechanism of Hyperbaric Oxygen for Glioblastoma and Predictive Indicators of Hyperbaric Oxygen Prolonging Survival in Glioblastoma Patients. (PubMed, Curr Med Sci)

HBO is beneficial for glioblastoma. Glioblastoma patients with these predictive indicators may prolong survival with HBO therapy. These potential therapeutic targets especially COL1A1, ADAMTS1 and PTBP3 deserve further validation.

2 months ago

Journal • PD(L)-1 Biomarker • IO biomarker

NF1 (Neurofibromin 1) • MYD88 (MYD88 Innate Immune Signal Transduction Adaptor) • PD-1 (Programmed cell death 1) • CXCR4 (Chemokine (C-X-C motif) receptor 4) • MDM4 (The mouse double minute 4) • CDK6 (Cyclin-dependent kinase 6) • CXCL12 (C-X-C Motif Chemokine Ligand 12) • CXCL13 (Chemokine (C-X-C motif) ligand 13) • CA9 (Carbonic anhydrase 9) • COL1A1 (Collagen Type I Alpha 1 Chain) • MMP9 (Matrix metallopeptidase 9) • PCNA (Proliferating cell nuclear antigen) • ADAMTS1 (ADAM Metallopeptidase With Thrombospondin Type 1 Motif 1) • CDC42 (Cell Division Cycle 42) • CDKN1B (Cyclin dependent kinase inhibitor 1B) • CXCR5 (C-X-C Motif Chemokine Receptor 5) • DDIT3 (DNA-damage-inducible transcript 3) • IGFBP3 (Insulin-like growth factor binding protein 3) • IGFBP5 (Insulin Like Growth Factor Binding Protein 5) • BAK1 (BCL2 Antagonist/Killer 1) • CDC25A (Cell Division Cycle 25A) • COL12A1 (Collagen Type XII Alpha 1 Chain) • COL8A1 (Collagen Type VIII Alpha 1 Chain) • EFEMP1 (EGF Containing Fibulin Extracellular Matrix Protein 1) • GNRP (Ras-Specific Guanine Nucleotide-Releasing Factor 1) • MAD2L1 (Mitotic Arrest Deficient 2 Like 1) • MCM2 (Minichromosome maintenance complex component 2) • RHOJ (Ras Homolog Family Member J) • NLRP2 (NLR Family Pyrin Domain Containing 2)

TP53 expression • CXCL12 expression • CDK6 expression • CDKN1B expression • PCNA expression

2ms

HKDC1 promotes autophagy and proliferation in pancreatic adenocarcinoma through interaction with PARP1 and poly(ADP-ribosyl)ation. (PubMed, Cell Signal)

HKDC1 plays a critical role in the malignant progression of PAAD by activating autophagy and promoting cell proliferation. Our findings suggest that targeting HKDC1 and its downstream signaling pathways may provide novel strategies for PAAD treatment.

2 months ago

Journal • PARP Biomarker

CCND1 (Cyclin D1) • PARP1 (Poly(ADP-Ribose) Polymerase 1) • PCNA (Proliferating cell nuclear antigen)

CCND1 expression • PCNA expression

3ms

Mechanism of emodin in treating hepatitis B virus-associated hepatocellular carcinoma: network pharmacology and cell experiments. (PubMed, Front Cell Infect Microbiol)

In vitro experiments demonstrated that emodin significantly downregulated the expression of HSP90AA1, MAPK3, XRCC1, PCNA, and SOD2, while significantly upregulating the expression of PTGS2 and GSTP1. This study, based on network pharmacology and molecular docking validation, suggests that emodin may exert therapeutic effects on HBV-related HCC by downregulating the expression of XRCC1, MAPK3, PCNA, HSP90AA1, and SOD2, and upregulating the expression of PTGS2 and GSTP1.

3 months ago

Journal

HIF1A (Hypoxia inducible factor 1, alpha subunit) • GSTP1 (Glutathione S-transferase pi 1) • PTGS2 (Prostaglandin-Endoperoxide Synthase 2) • TGFB1 (Transforming Growth Factor Beta 1) • PCNA (Proliferating cell nuclear antigen) • XRCC1 (X-Ray Repair Cross Complementing 1) • HSP90AA1 (Heat Shock Protein 90 Alpha Family Class A Member 1Heat Shock Protein 90 Alpha Family Class A Member 1) • MAPK3 (Mitogen-Activated Protein Kinase 3) • PACERR (PTGS2 Antisense NFKB1 Complex-Mediated Expression Regulator RNA) • SOD2 (Superoxide Dismutase 2)

HIF1A expression • PTGS2 expression • PCNA expression

3ms

Evodiamine inhibits proliferation and induces apoptosis of nasopharyngeal carcinoma cells via the SRC/ERBB2-mediated MAPK/ERK signaling pathway. (PubMed, J Transl Med)

When the MAPK/ERK signaling pathway was activated by epidermal growth factor (EGF), the expression levels of PCNA and XIAP increased, the cell proliferation index increased, and the apoptosis rate decreased in the EGF + EVO treatment group compared to treatment with EVO alone. These changes indicated that the inhibitory effect of EVO on proliferation and apoptosis of NPC cells was related to the down-regulation of SRC and ERBB2 expression, and further inhibition of the MAPK/ERK signaling pathway.

3 months ago

Journal

HER-2 (Human epidermal growth factor receptor 2) • CXCL8 (Chemokine (C-X-C motif) ligand 8) • STAT3 (Signal Transducer And Activator Of Transcription 3) • EGF (Epidermal growth factor) • HDAC1 (Histone Deacetylase 1) • PCNA (Proliferating cell nuclear antigen) • XIAP (X-Linked Inhibitor Of Apoptosis) • NOS3 (Nitric oxide synthase 3) • MAPK8 (Mitogen-activated protein kinase 8)

HER-2 expression • PCNA expression

3ms

Arctigenin inhibits the progression of colorectal cancer through epithelial-mesenchymal transition via PI3K/Akt/mTOR signaling pathway. (PubMed, PLoS One)

This is the first study to elucidate the mechanism by which arctigenin inhibits colorectal cancer metastasis through the PI3K-AKT-mTOR signaling pathway by suppressing the EMT process at the molecular level.

3 months ago

Journal • IO biomarker

BCL2 (B-cell CLL/lymphoma 2) • CDH1 (Cadherin 1) • MMP2 (Matrix metallopeptidase 2) • BAX (BCL2-associated X protein) • CASP3 (Caspase 3) • VIM (Vimentin) • CDH2 (Cadherin 2) • MMP9 (Matrix metallopeptidase 9) • PCNA (Proliferating cell nuclear antigen) • SNAI2 (Snail Family Transcriptional Repressor 2)

BCL2 expression • BAX expression • PCNA expression

3ms

High expression of ABL2 promotes gastric cancer cells migration, invasion and proliferation via the TGF-β and YAP signaling pathways. (PubMed, J Cancer)

Furthermore, GA-017 increased ABL2 expression in MGC-803 cells and counteracted the effects of si-ABL2 on cell migration, invasion and proliferation. These findings indicated that heightened ABL2 expression could activate TGF-β/SMAD2/3 and YAP signaling pathway, promoting epithelial mesenchymal transformation (EMT), and enhancing multiplication, metastasis, and invasion in GC cells.

3 months ago

Journal

MMP2 (Matrix metallopeptidase 2) • TIMP1 (Tissue inhibitor of metalloproteinases 1) • ABL2 (ABL Proto-Oncogene 2, Non-Receptor Tyrosine Kinase) • TGFB1 (Transforming Growth Factor Beta 1) • TIMP2 (TIMP Metallopeptidase Inhibitor 2) • MMP9 (Matrix metallopeptidase 9) • PCNA (Proliferating cell nuclear antigen)

KIM1 expression • TIMP1 expression • PCNA expression

3ms

Mechanism of Paeoniae Radix Rubra and Aconiti Lateralis Radix Praeparata herbal pair in promoting hepatocellular regeneration in chronic acute liver failure based on HGF/PI3K/Akt signaling axis (PubMed, Zhongguo Zhong Yao Za Zhi)

Furthermore, compared with the model group, the PRR-ALRP group showed upregulated expression of HGF and Gab1 proteins, as well as activation of PI3K and Akt phosphorylation. These findings suggest that PRR-ALRP herbal pair exerts anti-liver failure effects by alleviating hepatocyte inflammatory damage and promoting hepatocellular regeneration, and its specific regulatory mechanism may be related to the activation of the HGF/PI3K/Akt signaling pathway.

3 months ago

Journal

TNFA (Tumor Necrosis Factor-Alpha) • PCNA (Proliferating cell nuclear antigen) • IL1B (Interleukin 1, beta) • CCNB1 (Cyclin B1) • GAB1 (GRB2 Associated Binding Protein 1)

PCNA expression

3ms

YAP activation is robust to dilution. (PubMed, Mol Omics)

Together, this study provides an example case, where cytoplasmic dilution of a protein, for example through cell growth, does not limit a cognate cellular function. Here, that same proteins translocation into the nucleus.

3 months ago

Journal

CDK4 (Cyclin-dependent kinase 4) • PCNA (Proliferating cell nuclear antigen)

PCNA expression

3ms

Stat3 Inhibitors TTI-101 and SH5-07 Suppress Bladder Cancer Cell Survival in 3D Tumor Models. (PubMed, Cells)

Moreover, TTI-101 and SH5-07 target cancer stem cells by downregulating the expression of CD44 and CD133 in 3D models. This study provides the first evidence for the prevention of BCa with small-molecule inhibitors TTI-101 and SH5-07 via suppression of CSCs and STAT3 signaling.

3 months ago

Preclinical • Journal • PARP Biomarker

CCND1 (Cyclin D1) • CD44 (CD44 Molecule) • CASP3 (Caspase 3) • PCNA (Proliferating cell nuclear antigen) • CASP7 (Caspase 7)

CCND1 expression • CD44 expression • CD133 expression • PCNA expression

3ms

Preliminary Evidence of the Possible Roles of the Ferritinophagy-Iron Uptake Axis in Canine Testicular Cancer. (PubMed, Animals (Basel))

Sertoli cell tumors (SCTs) showed only NCOA4 expression. These preliminary findings highlight potential molecular targets for developing new anti-neoplastic treatments in canine testicular tumors.

3 months ago

Journal

NCOA4 (Nuclear Receptor Coactivator 4) • TFRC • PCNA (Proliferating cell nuclear antigen) • FTH1 (Ferritin Heavy Chain 1)

TFRC expression • PCNA expression

3ms

Targeting NPM1 inhibits proliferation and promotes apoptosis of hepatic progenitor cells via suppression of mTOR signalling pathway. (PubMed, Stem Cell Res Ther)

Targeting NPM1 inhibits proliferation and induces apoptosis in hepatic progenitor cells and BEL-7402 cells, thus serving as a potential therapy for cHCC-CCA.

3 months ago

Journal

NPM1 (Nucleophosmin 1) • IL6R (Interleukin 6 receptor) • PCNA (Proliferating cell nuclear antigen)

NPM1 expression • PCNA expression

NSC348884

4ms

Cannabidiol exhibits potent anti-cancer activity against gemcitabine-resistant cholangiocarcinoma via ER-stress induction in vitro and in vivo. (PubMed, BMC Complement Med Ther)

The results suggest that CBD exhibits potent anti-cancer activity against gemcitabine-resistant CCA in vitro and in vivo, in part via ER stress-mediated mechanisms. These results indicate that clinical explorative use of CBD on gemcitabine-resistant CCA patients is warranted.

4 months ago

Preclinical • Journal

ER (Estrogen receptor) • PCNA (Proliferating cell nuclear antigen) • ATF4 (Activating Transcription Factor 4)

PCNA expression

gemcitabine

7ms

Induction of Peroxiredoxin 1 by Hypoxia Promotes Cellular Autophagy and Cell Proliferation in Oral Leukoplakia via HIF-1α/BNIP3 Pathway. (PubMed, J Mol Histol)

In Prx1flox/flox:Cre+ mice, an increased expression of HIF-1α/BNIP3, LC3B and Beclin-1 was detected in epithelial hyperplasia tongue tissues compared to normal tissues. The current study suggests that Prx1 may promotes cell proliferation and autophagy in oral leukoplakia cells via the HIF-1α/BNIP3 pathway.

7 months ago

Journal

HIF1A (Hypoxia inducible factor 1, alpha subunit) • PRDX1 (Peroxiredoxin 1) • PCNA (Proliferating cell nuclear antigen) • BNIP3 (BCL2 Interacting Protein 3) • BECN1 (Beclin 1)

HIF1A expression • PCNA expression

7ms

The effects of anti-lung cancer in nude mice by a fully human single-chain antibody against associated antigen Ts7TMR between A549 cells and Trichinella spiralis. (PubMed, Artif Cells Nanomed Biotechnol)

Mechanistically, immunohistochemical staining revealed that the scFv down-regulated the expression of PCNA and VEGF in tumour tissues. Overall, this study found that anti-Ts7TMR scFv could inhibit A549 lung cancer growth by suppressing cell proliferation and angiogenesis, which may provide a new strategy for treating lung cancer.

7 months ago

Preclinical • Journal

PCNA (Proliferating cell nuclear antigen)

PCNA expression

7ms

Campothecin suppresses cell proliferation and migration in head and neck squamous cell carcinoma by blocking RAB27A-mediated phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway. (PubMed, J Physiol Pharmacol)

Reducing RAB27A expression enhanced the suppressive impacts of CPT on HNSCC cell viability (p<0.05 and p<0.01), migration (p<0.001) and invasion (p<0.01), these effects that were reversed upon treatment with L740Y-P in HNSCC cells (p<0.001). In summary, our study highlights the efficacy of CPT in HNSCC, demonstrating its influence on cell processes via the RAB27A-mediated PI3K/AKT pathway.

7 months ago

Journal

CDH1 (Cadherin 1) • PCNA (Proliferating cell nuclear antigen) • RAB27A (RAB27A, Member RAS Oncogene Family)

CDH1 expression • PCNA expression

7ms

ARHGAP17 Inhibits Hepatocellular Carcinoma Progression by Inactivation of Wnt/β-Catenin Signaling Pathway. (PubMed, Biochem Genet)

With overexpression of ARHGAP17 in HCC cells, the p-GSK3β/GSK3β decreased, while the p-β-catenin/β-catenin, Axin1 and APC increased. In conclusion, ARHGAP17 inhibits HCC progression by inactivating the Wnt/β-catenin signaling pathway.

7 months ago

Journal

CDH1 (Cadherin 1) • CDH2 (Cadherin 2) • PCNA (Proliferating cell nuclear antigen) • AXIN1 (Axin 1)

CDH1 expression • PCNA expression

8ms

AMPK regulates immature boar Sertoli cell proliferation through affecting CDK4/Cyclin D3 pathway and mitochondrial function. (PubMed, Theriogenology)

A high dose of estradiol (10 μM-6 h, showed a promotion of AMPK activation in a previous study) significantly inhibited SC ultrastructure, mitochondrial function, and proliferation-related pathways, while these adverse effects were weakened by Compound C treatment or miR-1285 mimic transfection. Our findings suggest that the activation and inhibition of AMPK induced by specific drugs or synthesized targeted miRNA fragments could regulate immature boar SC proliferative activity by influencing the CDK4/Cyclin D3 pathway and mitochondrial function; this helps to provide a basis for the prevention and treatment of male sterility in clinical practice.

8 months ago

Journal

CDK4 (Cyclin-dependent kinase 4) • CCND3 (Cyclin D3) • PCNA (Proliferating cell nuclear antigen) • CDKN1A (Cyclin-dependent kinase inhibitor 1A) • AMPK (Protein Kinase AMP-Activated Catalytic Subunit Alpha 1)

PCNA expression

dorsomorphin (Compound C)

8ms

Linaclotide in Treating Patients With Stages 0-3 Colorectal Cancer (clinicaltrials.gov)

P2, N=230, Active, not recruiting, Sidney Kimmel Cancer Center at Thomas Jefferson University | Trial completion date: Dec 2024 --> Jun 2025 | Trial primary completion date: Dec 2022 --> Jun 2025

8 months ago

Trial completion date • Trial primary completion date

MYC (V-myc avian myelocytomatosis viral oncogene homolog) • PCNA (Proliferating cell nuclear antigen) • AXIN1 (Axin 1)

PCNA expression

8ms

EIF3B stabilizes PCNA by counteracting SYVN1-mediated ubiquitination to serve as a promotor in cholangiocarcinoma. (PubMed, Aging (Albany NY))

Our findings underscored the potential of EIF3B as a therapeutic target for cholangiocarcinoma. Unraveling its functions holds promise for the development of more specific and effective targeted therapy strategies.

8 months ago

Journal

PCNA (Proliferating cell nuclear antigen) • CDKN1A (Cyclin-dependent kinase inhibitor 1A)

PCNA expression

8ms

Effects of silencing NLRP3 gene on proliferation of psoriasis-like HaCaT cells and expressions of cytokines. (PubMed, Cell Mol Biol (Noisy-le-grand))

Compared with negative control group, expressions of NLRP3 mRNA and protein, proliferation rate and clonal formation rate were decreased in NLRP3-RNAi group, percentage of cells in G0/G1 phase was increased, percentage of cells in S phase was decreased, expressions of Cyclin B1, CDK2, Ki67 and PCNA proteins were decreased, and levels of IL-17, IL-23, IL-6 and TNF-α were decreased. Silencing NLRP3 gene can inhibit the proliferation of psoriasis-like HaCaT cells, arrest cell cycle, inhibit the expressions of cell proliferation-related proteins and reduce levels of pro-inflammatory factors.

8 months ago

Journal

IL6 (Interleukin 6) • TNFA (Tumor Necrosis Factor-Alpha) • CDK2 (Cyclin-dependent kinase 2) • IL17A (Interleukin 17A) • IL23A (Interleukin 23 Subunit Alpha) • PCNA (Proliferating cell nuclear antigen) • NLRP3 (NLR Family Pyrin Domain Containing 3) • CCNB1 (Cyclin B1)

CDK2 expression • PCNA expression

8ms

Effect of salvia miltiorrhiza combined with roxadustat on wound healing of full-thickness skin defects in diabetic rats and its mechanism (PubMed, Zhonghua Shao Shang Yu Chuang Mian Xiu Fu Za Zhi)

At 14 d after injury, the protein expression level of EGFR in the wound tissue of rats in roxadustat+salvia miltiorrhiza group was significantly higher than the levels in the other 3 groups (with P values all 0.05). Roxadustat combined with salvia miltiorrhiza can promote the wound healing of full-thickness skin defects in diabetic rats by promoting blood vessel regeneration and reducing inflammatory response.

8 months ago

Preclinical • Journal

EGFR (Epidermal growth factor receptor) • IL6 (Interleukin 6) • TNFA (Tumor Necrosis Factor-Alpha) • HIF1A (Hypoxia inducible factor 1, alpha subunit) • CD31 (Platelet and endothelial cell adhesion molecule 1) • PCNA (Proliferating cell nuclear antigen) • IL1B (Interleukin 1, beta) • PECAM1 (Platelet And Endothelial Cell Adhesion Molecule 1)

EGFR expression • CD31 expression • HIF1A expression • VEGFA expression • IL6 expression • PCNA expression

Evrenzo (roxadustat)

8ms

Effects of Low-Intensity Pulsed Ultrasound on the Regulation of Free Fatty Acid Release in 3T3-L1 Cells. (PubMed, J Ultrasound Med)

LIPUS could promote the proliferation and differentiation of 3T3-L1 cells and inhibit TNF-α-induced lipolysis, indicating its potential as a therapy for mitigating lipotoxicity caused by decompensated adipocytes.

8 months ago

Journal

TNFA (Tumor Necrosis Factor-Alpha) • PCNA (Proliferating cell nuclear antigen) • PPARG (Peroxisome Proliferator Activated Receptor Gamma) • PLIN2 (Perilipin)

PLIN2 expression • PCNA expression

8ms

Identification of a potent PCNA-p15-interaction inhibitor by autodisplay-based peptide library screening. (PubMed, Microb Biotechnol)

Furthermore, the inhibition of PCNA interactions was investigated using p15, a PIP-box containing protein involved in DNA replication and repair. P3 inhibited the PCNA-p1551-70 interaction with a half maximal inhibitory activity of 16.2 μM, characterizing P3 as a potent inhibitor of the PCNA-p15 interaction.

8 months ago

Journal

PCNA (Proliferating cell nuclear antigen)

PCNA expression

8ms

Biomimetic MDSCs membrane coated black phosphorus nanosheets system for photothermal therapy/photodynamic therapy synergized chemotherapy of cancer. (PubMed, J Nanobiotechnology)

Herein, a photo-responsive multifunctional nanosystem was designed for cancer therapy, in which myeloid-derived suppressor cell (MDSC) membrane vesicle encapsulated decitabine-loaded black phosphorous (BP) nanosheets (BP@ Decitabine @MDSCs, named BDM)...Flow cytometry revealed significantly decreased infiltration of MDSCs and M2-macrophages along with an increased proportion of CD4+, CD8+ T cells as well as CD103+ DCs, suggesting a potentiated anti-tumor immune response. In summary, BDM realizes photothermal therapy/photodynamic therapy synergized chemotherapy for cancer.

8 months ago

Journal

CD8 (cluster of differentiation 8) • CD4 (CD4 Molecule) • CASP3 (Caspase 3) • HMGB1 (High Mobility Group Box 1) • CALR (Calreticulin) • ITGAE (Integrin Subunit Alpha E) • PCNA (Proliferating cell nuclear antigen)

PCNA expression

decitabine

8ms

GPR39-mediated ERK1/2 signaling reduces permethrin-induced proliferation of estrogen receptor α-negative cells. (PubMed, Ecotoxicol Environ Saf)

Knockdown of GPR39 partially impeded permethrin-induced cellular proliferation and altered the expression of proliferation marker protein PCNA and cell cycle-associated protein cyclin D1 via the ERK1/2 signaling pathway. These findings offer novel evidence for permethrin as an environmental breast cancer risk factor, displaying its potential to impact breast cancer cell proliferation via an estrogen receptor-independent pathway.

8 months ago

Journal

ER (Estrogen receptor) • CCND1 (Cyclin D1) • CHI3L1 (Chitinase 3-like 1) • PCNA (Proliferating cell nuclear antigen)

ER negative • CCND1 expression • PCNA expression

9ms

Laurencia johnstonii extract reverses early lesions in the K14E7HPV16 murine cervical carcinogenesis model. (PubMed, J Med Virol)

Differences of p < 0.05 were considered statistically significant through the whole study. Based on these results, we propose that the use of ELj could be an alternative for the treatment of cervical early lesions.

9 months ago

Preclinical • Journal

MIR21 (MicroRNA 21) • CASP3 (Caspase 3) • PCNA (Proliferating cell nuclear antigen) • MIR218 (MicroRNA 218)

PCNA expression

9ms

Inhibitory receptors of plasmacytoid dendritic cells as possible targets for checkpoint blockade in cancer. (PubMed, Front Immunol)

Based on this evidence, we propose that the regulation of IFN secretion by IRs may be regarded as an "innate checkpoint", reminiscent of the function of "classical" adaptive immune checkpoints, like PD1 expressed in CD8+ T cells, which restrain autoimmunity and immunopathology but favor chronic infections and tumors. However, we also point out that further work is needed to fully unravel the biology of tumor-associated pDCs, the neat contribution of pDC exhaustion in tumor growth following the engagement of IRs, especially those expressed also by other leukocytes, and their therapeutic potential as targets of combined immune checkpoint blockade in cancer immunotherapy.

9 months ago

Review • Journal • Checkpoint inhibition • Checkpoint block

CD8 (cluster of differentiation 8) • PD-1 (Programmed cell death 1) • HAVCR2 (Hepatitis A Virus Cellular Receptor 2) • PCNA (Proliferating cell nuclear antigen)

PD-1 expression • CD8 expression • PCNA expression

9ms

Dendrobium officinale Polysaccharide (DOP) inhibits cell hyperproliferation, inflammation and oxidative stress to improve keratinocyte psoriasis-like state. (PubMed, Adv Med Sci)

DOP inhibits keratinocyte hyperproliferation, inflammation and oxidative stress to improve the keratinocyte psoriasis-like state.

9 months ago

Journal

CCND1 (Cyclin D1) • IL6 (Interleukin 6) • TNFA (Tumor Necrosis Factor-Alpha) • IL17A (Interleukin 17A) • IL23A (Interleukin 23 Subunit Alpha) • PCNA (Proliferating cell nuclear antigen) • IL1B (Interleukin 1, beta) • IL22 (Interleukin 22)

CCND1 expression • IL6 expression • PCNA expression

9ms

Anti-proliferative and apoptotic effect of cannabinoids on human pancreatic ductal adenocarcinoma xenograft in BALB/c nude mice model. (PubMed, Sci Rep)

In this study, the anti-proliferative and apoptotic effect of cannabinoid solution (THC:CBD at 1:6) at a dose of 1, 5, and 10 mg/kg body weight compared to the negative control (sesame oil) and positive control (5-fluorouracil) was investigated in human PDAC xenograft nude mice model...These findings suggest that cannabinoid solution (THC:CBD at 1:6) could inhibit proliferation and induce apoptosis in human PDAC xenograft models. Cannabinoids, including THC:CBD, should be further studied for use as the potent PDCA therapeutic agent in humans.

9 months ago

Preclinical • Journal • IO biomarker

BCL2 (B-cell CLL/lymphoma 2) • BAX (BCL2-associated X protein) • CASP3 (Caspase 3) • CASP8 (Caspase 8) • PCNA (Proliferating cell nuclear antigen)

BCL2 expression • PCNA expression

5-fluorouracil