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over2years
B7H3-targeted tri-specific killer engagers deliver IL-15 to NK cells but not T-cells, and specifically target solid tumors as a pan-tumor antigen strategy mediated through NK cells (ESMO 2022)
As proof of concept, a clinical trial of GTB-3550 (a CD33-targeted Tri-specific Killer Engager [TriKE] in AML) induced endogenous NK cell expansion and activation in refractory AML patients. Here we developed GTB-5550 (a B7H3 TriKE) as a novel dual camelid (cam) TriKE containing WT IL-15 and comprised of two cam engagers: targeting CD16 on NK cells and B7H3 on multiple solid tumors...Conclusions B7H3 TriKE delivers an NK cell specific IL-15 signal to expand NK cells and is highly specific against a broad array of cancers. Clinical manufacturing is underway with an IND planned to open clinical trials in 2023 in a number of solid tumors and multiple myeloma.
Pan tumor
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CD276 (CD276 Molecule) • CD33 (CD33 Molecule) • LAMP1 (Lysosomal Associated Membrane Protein 1) • IL15 (Interleukin 15)
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GTB-5550 • GTB-3550 TRIKE
over2years
GTB-3550 Tri-Specific Killer Engager (TriKE®) for High Risk Hematological Malignancies (clinicaltrials.gov)
P1/2, N=12, Terminated, GT Biopharma, Inc. | N=60 --> 12 | Trial completion date: Aug 2025 --> Sep 2021 | Active, not recruiting --> Terminated | Trial primary completion date: Feb 2024 --> Aug 2021; Development of GTB-3550 halted due to development of the second generation camelid nanobody TriKE drug product, GTB-3650.
Enrollment change • Trial completion date • Trial termination • Trial primary completion date
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CD33 (CD33 Molecule) • NCAM1 (Neural cell adhesion molecule 1)
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GTB-3550 TRIKE
over2years
GTB-5550 (cam16-IL15-camB7H3) trispecific killer engager (TriKE®) drives natural killer cell activation and antibody dependent cellular cytotoxicity against head and neck squamous cell carcinomas (AACR 2022)
NK cell degranulation and IFN-gamma production of GTB-3550-treated samples were higher compared to that of control samples treated with B7H3 single domain or IL-15 alone. Ongoing experiments will evaluate functionality of GTB-5550 on FA patient samples as well as in spheroid assays. Taken together, this data shows that a GTB-5550 is able to drive NK cell activity against B7H3-expressing HNSCC cells, which presents potential for a B7H3-targeted TriKE to be used to be implemented clinically to treat HNSCC or FA-HNSCC patients.
IO biomarker
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IFNG (Interferon, gamma) • CD276 (CD276 Molecule) • FANCA (FA Complementation Group A) • CD28 (CD28 Molecule) • IL15 (Interleukin 15)
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CD276 expression
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GTB-5550 • GTB-3550 TRIKE
3years
GTB-3550 Tri-Specific Killer Engager (TriKE®) for High Risk Hematological Malignancies (clinicaltrials.gov)
P1/2, N=60, Active, not recruiting, GT Biopharma, Inc. | Recruiting --> Active, not recruiting
Enrollment closed
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CD33 (CD33 Molecule) • NCAM1 (Neural cell adhesion molecule 1)
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GTB-3550 TRIKE
over3years
[VIRTUAL] GTB-3550 tri-specific killer engager safely activates and delivers IL-15 to NK cells, but not T-cells, in immune suppressed patients with advanced myeloid malignancies, a novel paradigm exportable to solid tumors expressing Her2 or B7H3 (ESMO 2021)
GTB-3550 TriKE given as a monotherapy safely induced a sustained functional expansion of endogenous NK cells with anti-tumor activity in advanced AML and MDS patients treated with at least 25 mcg/kg/day. Second generation solid tumor TriKE therapeutics against HER2 and B7H3 will be tested clinically next year.
Clinical
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HER-2 (Human epidermal growth factor receptor 2) • CD8 (cluster of differentiation 8) • CD276 (CD276 Molecule) • CD33 (CD33 Molecule) • CD4 (CD4 Molecule)
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HER-2 expression
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GTB-3550 TRIKE
4years
CD33-Targeted Therapies: Beating the Disease or Beaten to Death? (PubMed, J Clin Pharmacol)
Gemtuzumab ozogamicin was the first and only CD33-directed antibody-drug conjugate to be US Food and Drug Administration approved for AML...Promising new strategies include cellular therapy mechanisms and linker molecules. This is an exciting target that requires a considerable amount of precision to yield clinical benefit.
Review • Journal
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CD33 (CD33 Molecule)
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Mylotarg (gemtuzumab ozogamicin) • IMGN779 • vadastuximab talirine (SGN-CD33A) • vixtimotamab (AMV564) • AVE9633 • GTB-3550 TRIKE • eluvixtamab (AMG 330)