MIR96 (MicroRNA 96)

The identification and validation of biomarkers reflecting this continuum could enable the establishment of molecular margins-improving risk assessment, reducing local recurrence, and advancing personalized oncologic surgery in HNSCC. Standardizing definitions and sampling protocols for "normal adjacent tissue" remains essential for future translational research.

Among 33 inflammatory polyps, 24 scored negative, as well as 64/70 normal contralateral mucosa and one fungus ball. Therefore, excluding benign/borderline tumours, the detected sensitivity was 85.7% and specificity 85.6% (AUC: 0.878).

The assay accomplishes rapid (<45 min) and sensitive detection of miR-21, miR-155, and miR-96 and can distinguish breast-cancer patients from postoperative recovery subjects. These results demonstrate the potential of this biosensor for real-time health monitoring and POCT miRNA analysis, particularly for breast-cancer diagnosis.

ERα can function as aTF, modulating the expression of various circRNAs with differential expression in HCC. Through this regulation, it modulates the circRNA/miRNA/SMADs network, thereby inhibiting the progression of HCC.

The FOXO1 and ADAMTS9-AS2 genes might act as molecular suppressors in the cell growth pathways. Furthermore, miR-96-5p is suggested as an oncogenic miRNA in the LSCC.

The assay overcomes common challenges associated with multiple miRNAs detection, particularly in liquid biopsy samples (e.g., multiple pipetting issues, increased consumption of sample for multiple assessment, extended TAT for complete profiling) and provides robust and accurate detection, demonstrating potential for real-time patient monitoring and prognostication in HNC.

Additionally, we found eccDNA carrying full protein-coding genes that are overexpressed in transcriptional analyses. Our findings suggest that small eccDNAs with miRNA genes are functional and contribute to the tumorigenesis of renal cell carcinoma.

The C/T genotype of rs11614913 in MIR196A2, and C/A genotype and A allele of rs2682818 in MIR618, are associated with a protective effect against BrC in women from western Mexico.

This strategy demonstrates high specificity in SKOV3 cells, and its modular design enables rapid adaptation to other miRNA combinations (e.g., miR-21/miR-155) through DNA sequence reprogramming, offering a versatile platform for multi-tumor subtyping. The approach provides new perspectives for intelligent, portable biosensing and bioanalytical applications.

Evaluation of miRNAs commonly deregulated between the three groups showed 14 shared upregulated miRNAs (miR-429, miR-425-5p, miR-200c-3p, miR-200c-5p, miR-200b-3p, miR-200a-3p, miR-183-5p, miR-182-5p, miR-141-5p, miR-141-3p, miR-96-5p, miR-93-5p, miR-10a-5p, miR-10a-3p) with a progressive increase in expression levels, from ovarian EMS to TL and ultimately to ECOC. The identified miRNA expression profiles associated with the progression from ovarian EMS, TL and ECOC provide valuable insights into the molecular progression from benign to malignant lesions and could represent potential biomarkers for the early detection of ECOC.