MIR328 (MicroRNA 328)

Mechanistic evaluation revealed that circFOXK2 upregulates PKP3 by acting as an miR-328-5p sponge. The findings demonstrate that circFOXK2 contributes to NSCLC tumorigenesis via modulation of the miR-328-5p/PKP3 pathway, identifying this signaling axis as a potential therapeutic target in NSCLC.

Specifically, we highlight the modulatory roles of miRNA in cancer cell survival and proliferation (miR-28, miR-30b/c), invasion and metastasis (miR-218, miR-182), neoangiogenesis (miR-29c), metabolic reprogramming (miR-124), and therapy resistance (miR-378, miR-328, miR-1244). The broad implications of miRNAs in lung cancer underline their potential real-world utility, as these entities can function as biomarkers for prognosis/diagnosis and even future therapeutic targets or agents.

We also discuss miRNAs (such as miR-519a-3p, let-7e, miR-216b), which affect responses to OvCa therapy by targeting PARP1 (Poly(ADP-Ribose) Polymerase-1). Finally, we also discuss why, despite the identification of multiple miRNAs capable of regulating DNA repair genes, as well as those involved in the response to therapy, no miRNA-based drugs have been approved for OvCa treatment in clinics.

Additionally, our investigations revealed that RH significantly upregulated miR-328-3p expression, resulting in the downregulation of TCF7L2 protein levels in osteosarcoma cells. Our findings indicate that miR-328-3p/TCF7L2/FABP4 axis is vital for the anticancer effects of RH and may be a potential therapeutic agent for osteosarcoma treatment.

For MDS, DAI-CCNA2 was simulated in comparison with CCNA2-Olaparib (OLA), an approved drug for breast cancer. MDS results verified DAI (the proposed drug) to be a potential candidate to combat breast cancer. Identification of druggable polyphenols using such a comprehensive in-silico approach can aid in providing a novel therapeutic strategy to combat the drawbacks associated with conventional therapies that can be further validated in an experimental setup.

It could be concluded that miR-34a or miR-328 could effectively increase radiation- or DOX-induced cell apoptosis by negatively regulating Bcl-2 or ABCG2 expression levels in BCSCs, respectively. Hence, ectopic expression of these miRNAs could sensitize BCSCs to irradiation and DOX treatment.

Ovarian cancer cells with different invasive capabilities secrete distinct exosomes. Exosomes from highly invasive cells enhance these traits in less aggressive cells via hsa-miR-328-3p, which targets Raf1, disrupts the mTOR pathway, and promotes autophagy. This study highlights exosomes as carriers of hsa-miR-328-3p, mediating intercellular communication and autophagy to influence ovarian cancer cell heterogeneity.

The study contributes to the early prediction of lung cancer by identifying potential biomarkers that could enhance early diagnosis and pave the way for practical clinical applications in the future. Integrating DEGs and machine learning-derived significant genes for PPI analysis offers a robust approach to uncovering critical molecular targets for lung cancer treatment.

In addition, PCa patients with high miR-328-3p had a better prognosis than those with low miR-328-3p. Briefly, this study highlights the clinical and biological role of miR-328-3p as a tumor suppressor miRNA in PCa and explores the downstream mechanisms of miR-328-3p.

Taken together, circEIF3I elevated NCAPH expression by sponging miR-328-3p, thereby promoting CRC metastasis. These findings suggest that the circEIF3I/miR-328-3p/NCAPH axis represents a novel therapeutic target for CRC.

Our study has advanced our understanding of the roles of miR-328-3p and HMOX1 in HCC, demonstrating the inhibitory effect of miR-328-3p on the oncogenic activity of HMOX1. Hence, these results revealed the function of miR-328-3p and a novel mechanistic pathway for HCC and suggested the potential therapeutic targeting of miR-328-3p and HMOX1 for HCC intervention strategies.

Compared with those of the ox-LDL+miR-328-3p+pcDNA co-transfection group, the IGF2 protein level was significantly increased (P<0.05) and cell activity was significantly decreased (P<0.05) in the ox-LDL+miR-328-3p+IGF2 co-transfection group, while the apoptosis rate, cleaved cas-3 protein level, and the levels of TNF-α, IL-6, and IL-1β were significantly elevated (P<0.05). miR-328-3p inhibits ox-LDL-induced apoptosis and inflammatory in coronary artery endothelial cell injury through targeted negative regulation of IGF2.