MIR141 (MicroRNA 141)

In summary, our study highlights the pivotal role of miRNA biomarkers in shaping the landscape of T cell dysfunction across cancers. These miRNAs serve as both prognostic indicators and predictive tools, enabling accurate classification of TCD severity and offering new avenues for therapeutic exploration and patient stratification in cancer immunotherapy.

We further explored the translational potential of miRNA-based liquid biopsies (e.g., miR-141-3p and miR-34a) for diagnosing and prognosticating bone metastasis in breast, prostate, and lung cancers. Finally, this article looks ahead at how integrating multi-omics data with AI predictive models can overcome current delivery and safety challenges, advancing miRNA research towards the ultimate goal of precision medicine.

The developed method reproduced the expected differential miR-21 patterns across cancer and normal cell lines using cell-derived RNA extracts, consistent with stem-loop RT-qPCR. Notably, the method requires only a single hairpin probe per target miRNA and operates in a label-free format, enabling streamlined multiplex readout with minimal mutual interference. This platform offers a practical route toward efficient multiplex miRNA profiling in bioanalytical and diagnostic settings.

The platform also demonstrated strong selectivity in human urine spiked with interfering miRNAs. Owing to its modular probe design, this system is readily adaptable to diverse nucleic acid targets, offering a rapid, sensitive, and field-deployable solution for early cancer screening and point-of-care diagnostics.

Fundamental molecular processes underlying bladder cancer pathogenesis include cell cycle control, signal transduction, and genomic stability. These findings provide insight into the molecular regulatory landscape of MIBC and highlight potential targets for diagnostic and prognostic applications.

Conversely, the downregulation of miR-138 appeared to be a negative factor. Additionally, flow cytometry results demonstrated an increase in the rates of apoptosis and necrosis under the employed electromagnetic fields.

Furthermore, members of the miR‑200 family have potential as diagnostic and prognostic biomarkers and are closely linked to chemotherapy resistance. The present review aims to provide novel insights and a theoretical foundation for the diagnosis, treatment, and deeper investigation of BC by comprehensively examining the functional mechanisms of the miR‑200.

Furthermore, we identified miR-141 as a negative regulator of NPY1R, highlighting its potential as a therapeutic agent. Collectively, our results identified the UHRF1/NPY1R regulatory axis as a critical epigenetic mechanism in intestinal inflammation and underscored its dual promise for IBD diagnostics and therapy.

Although promising, clinical implementation of miRNA-based assays requires further validation, standardization of protocols, and large-scale prospective studies. Harnessing circulating miRNAs could usher in a new era of precision oncology for PCa, improving early diagnosis, prognostication, and real-time therapeutic guidance.

This review synthesizes the current evidence on the ncRNA-mediated regulatory networks that influence CRC radiosensitivity, emphasizing their potential as therapeutic targets to overcome RT resistance and improve outcomes in advanced CRC. By bridging mechanistic insights with clinical applications, this work aims to guide future research and the implementation of precision RT strategies.

We established the new radiotranscriptomic signatures specifically optimized for differentiating T3b stage from T2c stage by decoding stage-specific imaging-genomic crosstalk. This new approach may overcome TNM staging limitations.

Among the miRNA alterations associated with stage I adenocarcinoma in nonsmokers, we found alterations in all 5 miR-200 family members (miR-200b, miR-200a, miR-141, miR-429, and miR-200c). Our identification of miRNA alterations associated with early-stage lung adenocarcinoma in nonsmokers, especially alterations in the miR-200 family, suggests that these miRNAs may play a unique role in the early stages of lung carcinogenesis and progression in nonsmokers and that they may be useful as markers for the early detection of lung cancer.