ET206

To better understand the migration of these cells and possibly influence their migration, NSG mice were conditioned with agents to clear BM cellular compartments, i.e., busulfan or total body irradiation (TBI), or promote T-cell migration to inflamed BM, i.e., incomplete Freund's adjuvant (IFA), prior to administering γδ T cells...Using an established neuroblastoma NSG mouse model, we show that intratumorally-injected γMSCs increase the homing of γδ T cells to this tumor. These studies provide insight into the migration of serum-free, ex vivo-expanded Vγ9Vδ2 T cells in NSG mice, which is critical to understanding the fundamental properties of these cells.

Neuroblastoma (NB) is the most common cancer in infants and represents approximately 15% of cancer-related deaths. We initiated a Phase I study using allogeneic ex vivo expanded gamma delta (?d) T cells for relapsed NB (NCT05400603), and have extended the ?d T cell platform to include proprietary cell-specific transgene expression vector designs and gene transfer systems for eBite expression, which generate our Allogeneic Donor Expanded and Programmed T (ADEPT) cells.