FUT3 (Fucosyltransferase 3)

Moreover, we found that fucosylation of GRP78 triggers downstream ER stress signaling pathways and activates the PERK/ATF4/STC2 pathway, leading to enhanced glucose deficiency tolerance in CRC cells. Overall, our study highlights the significant role of FUT3 in the fucosylation of GRP78, which is crucial for the survival and proliferation of CRC cells in a glucose-deficient microenvironment.

RNA sequencing analysis identified significantly upregulated genes in Gemcitabine (Gem)-resistant cells of PDAC(ASPC-1/Gem)...Mechanistically, FUT3 bound to β-1,3-N-acetylglucosaminyltransferase 3 (B3GNT3) to activate Nuclear Factor-kappa B (NF-κB) signal transduction, driving autophagy, progression and chemoresistance. Our results reveal that FUT3 interacts with B3GNT3 and activates the NF-κB signaling, amplifying autophagic activity to drive PDAC progression and confer chemoresistance.

Malignancies with elevated CA19-9 expression, such as pancreatic, biliary, and ovarian cancers, are associated with poor prognosis and limited response to current therapies. This study presents a tumor marker-guided strategy for boron neutron capture therapy (BNCT) by leveraging CA19-9 glycan biology to enable selective tumor targeting via fucose-BSH, a novel boron compound. Through transcriptomic data mining and preclinical validation, fucose-BSH demonstrated LAT1-independent boron delivery, potent BNCT-mediated cytotoxicity, and tumor-specific accumulation in CA19-9-positive models. These findings support a precision chemoradiation approach that addresses a critical gap in BNCT applicability, offering a clinically actionable pathway for patient stratification and therapeutic development in CA19-9-expressing cancers.

SLC27A2 mRNA expression was elevated in tumor tissues relative to adjacent noncancerous tissues, whereas the mRNA expression levels of the other four genes were decreased. This study reveals the important role of PTMs in BC prognosis and provides new perspectives for the prognostic assessment of BC patients as well as personalized treatment.

Immunohistochemical validation confirmed that B3GNT3 is highly expressed in real-world cases of PAAD, COAD, LUAD, UCEC, and OV. Pan-cancer analysis of B3GNT3 reveals complex regulatory mechanisms across different types of cancer, emerging as a novel biomarker for cancer diagnosis and prognosis, and offers new avenues for targeted therapeutic development.

In HT-29 cells, both RA concentrations suppressed MUC1 extracellular domain; C1GalT1 was diminished by 200 μM RA, Gal-3 and pAkt by 400 μM RA. The data suggest possible benefit of RA as the agent supporting colon cancer treatment.

Genetic testing is ready for prime time in PDAC screening. It stratifies patients into low-risk (no surveillance) and high-risk (surveillance warranted) groups, improving early detection, outcomes, and cost-effectiveness, thus transforming PDAC prognosis through targeted intervention.

ST6Gal-I rs2239611 was a potential genetic biomarker for lung cancer. Areca nut chewing, cigarette smoking, alcohol drinking interacts with glycosyltransferase gene polymorphisms (FUT2 rs1047781 and ST6Gal-I rs2239611), increasing lung cancer risk-a novel finding given the lack of prior studies on this combination.

Our findings demonstrate that elevated CA19-9 levels during immunotherapy are associated with poor survival outcomes in HCC patients. These findings highlight the crucial role of CA19-9 in shaping the tumor immune environment, particularly through its effect on macrophage polarization, and suggest that targeting CA19-9 may improve immunotherapy outcomes.

In contrast, C57BL/6 and FcγR-humanized C57BL/6 mice both have endogenous IgG that occupy their FcγR (murine for the former and human for the latter), precluding interactions with radioimmunoconjugates. Ultimately, these data suggest that understanding the interplay between radiolabeled antibodies and FcγR is critical during the preclinical evaluation of radioimmunoconjugates.

This study established a nine-DE_GRG-based prognostic signature, which independently predicted ccRCC prognosis. This finding emphasizes that GRGs are stratification factors for the precise prognosis of ccRCC.