In this study, we show that CRISPR/Cas9-mediated loss of E7 from HPV + OPSCC cells, SCC2 and SCC104, restored cGAS-STING responses. Future work could involve HPV oncogene targeting leading to HPV + OPSCC tumour regression and that the combined use of STING agonists would induce favourable tumour clearance by activating appropriate anti-tumour responses.

over 4 years ago

Journal

STING (stimulator of interferon response cGAMP interactor 1)

CEP-37250

over4years

BCCIPβ facilitates p53 ubiquitination via binding with E6 protein in high-risk HPV positive head and neck squamous cell carcinoma. (PubMed, Biochem Biophys Res Commun)

HPV16-positive UM-SCC-47 (SCC47) and UM-SCC-104 (SCC104) and HPV-negative SCC-9 (SCC9) and UM-SCC-1 (SCC1) cell lines were used as in vitro cell models...The presence of HPV E6 turned BCCIPβ from a p53 stabilizer to a ubiquitination facilitator. This mechanism helps explain why BCCIPβ acted as a tumor suppressor in HPV-negative HNSCC but exerted oncogenic function in HPV16-positive HNSCC.

over 4 years ago

Journal • BRCA Biomarker

BRCA2 (Breast cancer 2, early onset) • CDKN1A (Cyclin-dependent kinase inhibitor 1A)

CDKN2A negative

CEP-37250

over4years

[VIRTUAL] 4NQO shows formation of yH2AX foci, oxidative base damage and activates DNA repair pathway preferentially in HPV positive HNSCC cells (AACR-II 2020)

Our preliminary study shows for the first time that HPV E6/E7 oncogenes promote DNA repair in NOK and HPV positive HNSCC cells in response to 4NQO treatment. This could explain why HPV associated HNSCC have a better prognosis irrespective of smoking history.

over 4 years ago

BRCA Biomarker

BRCA1 (Breast cancer 1, early onset) • RAD51 (RAD51 Homolog A) • CHEK1 (Checkpoint kinase 1)

CEP-37250