CD9 (CD9 Molecule)

These effects required both Na+/K+-ATPase-dependent endocytosis and autophagy, as rapamycin-induced autophagy alone did not remove surface CD63. Our findings reveal a previously unrecognized mechanism in which cardiac glycoside regulates EV cargo composition by coupling Na+/K+-ATPase-mediated endocytosis with autophagy. Given that endogenous and therapeutic cardiac glycosides are implicated in cardiovascular and cancer biology, this mechanism may broadly influence EV-mediated intercellular communication and represent a potential target for modulating EV functions.

Elevated PDGF-B reversely stimulates TGFBI production in macrophages, which creates a positive feedback loop. This TGFBI-mediated interaction between HSCs and macrophages remodels the profibrotic microenvironment to promote liver fibrosis, identifying a potential therapeutic target.

P=N/A, N=60, Not yet recruiting, Tianjin Third Central Hospital; Tianjin Third Central Hospital

Compared with non-APL-AML patients, APL patients (PML-RARα (S-type) and PML-RARα (L-type)) exhibit unique immunophenotypic changes. The expression frequencies of CD56, CD2, CD34, and CD200 in leukemia cells are significantly correlated with the OS of APL patients, and the high expression of these indicators before treatment may be an adverse prognostic factor for APL patients.

RNA-seq revealed notable inter-laboratory variation. These findings highlight the variability across measurement platforms and emphasize the need for reproducible methods to support NIST's mission of developing reliable EV reference materials.

Among the previously identified EV cargo, HIF1a and KAT2B were found to be expressed in RB tumors, indicating their potential as biomarkers. KAT2B, previously identified as EV cargo, showed expression in tumor tissue, validating its biomarker potential.

The system presented herein represents a significant advancement in exosome purification, offering a robust and automated platform for liquid biopsy-based cancer research and clinical applications. This innovation holds promise for cancer diagnosis, prognosis, and monitoring through non-invasive biomarkers.

Conversely, Jag1 overexpression augments TIC self-renewal. Thus, Jagged1-mediated Notch signaling controls a hybrid-EMT state that is a defining feature of TICs in PDAC.

This proof-of-concept study suggests an impact of ABT on the urinary EV-microRNA cargo and yields comprehensive findings into surface markers of urinary EVs. While the adoption of urinary EV-associated microRNAs in routine doping tests requires further exploration, these data serve as a valuable basis for a variety of subsequent investigations.

Importantly, the combined biomarker panel (alpha-fetoprotein, miR-27a-3p, and miR-493-3p) achieved markedly improved performance (area under the curve = 0.943), outperforming alpha-fetoprotein alone (area under the curve = 0.828).ConclusionsSerum exosomal miR-27a-3p and miR-493-3p exhibit strong diagnostic potential and are associated with forkhead box O1 and serine/arginine-rich splicing factor 11. These findings highlight their promise as complementary biomarkers and provide new insight into exosome-mediated molecular regulation in hepatocarcinogenesis.

These findings highlight the potential of preoperative quantification of CD44v6-positive EVs as a biomarker for risk stratification and treatment optimization in PDAC.

Our findings demonstrate that EV composition remains stable in Protein Plus BCT compared to EDTA and ACD-A tubes, with a small number of exceptions. This insight into EV stability in blood specimens is crucial for advancing EV biomarker research in neurological disorders, including Alzheimer's disease, potentially enhancing diagnostic accuracy and therapeutic strategies.