...in the 22 092 cells, PTEN was not expressed and AKT phosphorylation was elevated (Figure ​(Figure4A).4A). The allosteric AKT inhibitor MK2206 inhibited AKT phosphorylation in 22 092 cells (Figure ​(Figure4B)4B) and 22 092 cells were more sensitive to MK2206 than A375 cells (Figure ​(Figure4C).4C). Taken together, our data show that, in the tumours from this patient, ERK activity was elevated due to the GNAQ P209 mutation, and AKT activity was elevated by loss of PTEN expression.