The effect of PHI-501 on anchorage independent growth and cell proliferation compared to vemurafenib or belvarafenib (pan-RAF inhibitor) were tested in A375 (BRAF V600E), C8161 (BRAF G464E) and SK-MEL-2 (NRAS Q61R) melanoma cell lines...PHI-501 showed 3- to 119-fold more effective growth inhibition than other RAF inhibitors, vemurafenib and belvarafenib, in SK-MEL-2 (GI50: 0.15 µM for PHI-501 vs 7.13 µM and 0.37 µM, respectively) and C8161 (GI50: 0.33 µM for PHI-501 vs 39.56 µM and 2.10 µM, respectively) melanoma cell lines...