We determined that the sensitivity of detection and cytotoxic killing of 5T4 expressing cells by OXB-302 cells exceeds detection by flow cytometry methods....Together, these data evidence the fact that 5T4-targeted CAR-T cells show specificity and efficient of cell killing. This suggests that OXB-302 could successfully kill 5T4-expressing leukaemic blasts from AML patients and further eliminate 5T4-expressing leukaemic stems cells, which would help to prevent relapse.