Cohort II included 33 patients who failed first-line therapies and further received anti-PD-1 therapy, prior to which needle biopsies were obtained....a high expression of CD274 was associated with response to ICIs and with a better PFS compared to patients with low CD274 expression (P value < 0.01). Further, among patients with high CD274 expression, a high GZMA expression was associated with a trend towards better PFS (P value = 0.06)...The data suggests that PD-L1and GZMA mRNA expression may predict the prognosis and response to immune checkpoint blockade therapies in NSCLC patients...