A similar experiment was conducted with the MGMT-expressing, MSI CRC cell line HCT116 treated with TMZ, and a similar result was observed as far as a lack of T cell killing enhancement with TMZ alone (Figure 5). Together, these results suggest that TMZ + ICI significantly enhances TALL-104-mediated killing of MSS, MGMT-expressing CRC cell lines, but each treatment alone had no effect....Our results indicate that TMZ sensitizes MSS, MGMT-expressing CRC cells to ipilimumab + nivolumab ICI.