LB was performed at diagnosis (n = 10), at each radiologic evaluation (n = 75) and at the time of progressive disease (PD) (n = 34) in 35 FISH/RNAseq confirmed ALK+ NSCLC patients, treated with ALK-TKI....ALK resistance mutations were detected in samples collected at PD under crizotinib (L1196M+C1156Y), ceritinib (F1174L), brigatinib (F1174V, F1174L+G1202R, D1203N, R1192P) and lorlatinib (F1174L+G1202R).